Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …

CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA
cleavage,,–, and the underlying mechanisms by which Cas9 recognizes mismatches are …

Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important
forage crops, is challenging due to the lack of a reference genome and an efficient genome …

CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …

Gene editing stands for the methods to precisely make changes to a specific nucleic acid
sequence. With the recent development of the clustered regularly interspaced short …

The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional
repressor, but inefficiencies in target-gene silencing have limited its utility. Here we describe …

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target
locus as the first step to gene correction,. Although most genetic diseases arise from point …

CRISPR (clustered regularly interspaced short palindromic repeat) systems have been
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …