The pandemic of COVID-19 creates an imperative need for sensitive and portable detection
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …

Control of CRISPR/Cas12a trans-cleavage is crucial for biosensor development. Here, we
show that small circular DNA nanostructures which partially match guide RNA sequences …

Abstract Aflatoxin B1 (AFB1) contamination severely threatens human and animal health, it
is thus critical to construct a strategy for its rapid, accurate, and visual detection. Herein, a …

Japanese encephalitis (JE), a mosquito-borne zoonotic disease caused by the Japanese
encephalitis virus (JEV), poses a serious threat to global public health. The low viremia …

How to realize fM-level detection of viral DNA without target amplification is a challenge.
Traditionally, preamplification of low-abundant DNA targets is a prerequisite, which not only …

Salivary miRNA-31 is a reliable diagnostic marker for early-stage oral squamous cell
carcinoma (OSCC), but accurate detection of miRNA-31 in saliva samples is a challenge …

The trans-cleavage properties of Cas12a make it important for gene editing and disease
diagnosis. In this work, the effect of spatial site resistance on the trans-cleavage activity of …

The CRISPR/Cas13 nucleases have been widely documented for nucleic acid detection.
Understanding the intricacies of CRISPR/Cas13's reaction components is pivotal for …

Tuberculosis is a highly infectious bacterial disease caused by Mycobacterium tuberculosis.
The spread of this agent has caused serious health problems worldwide, and the rapid and …

Inflammatory bowel disease (IBD) is a chronic disorder associated with inflammation in the
gastrointestinal tract, leading to a range of debilitating symptoms. Fecal calprotectin is an …