Methodological guidelines to study extracellular vesicles

FAW Coumans, AR Brisson, EI Buzas… - Circulation …, 2017 - Am Heart Assoc
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …

Extracellular vesicles as biomarkers in liver diseases: a clinician's point of view

S Thietart, PE Rautou - Journal of hepatology, 2020 - Elsevier
Extracellular vesicles are membrane-bound vesicles containing proteins, lipids, RNAs and
microRNAs. They can originate from both healthy and stressed cells, and provide a …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Standardization of sample collection, isolation and analysis methods in extracellular vesicle research

KW Witwer, EI Buzás, LT Bemis, A Bora… - Journal of …, 2013 - Taylor & Francis
The emergence of publications on extracellular RNA (exRNA) and extracellular vesicles
(EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease …

Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material

A Görgens, M Bremer, R Ferrer-Tur… - Journal of …, 2019 - Taylor & Francis
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and
pathophysiological conditions and are increasingly recognised as potential biomarkers …

Emerging role of extracellular vesicles in inflammatory diseases

EI Buzas, B György, G Nagy, A Falus… - Nature Reviews …, 2014 - nature.com
The discovery that submicron-sized extracellular vesicles (EVs) are generated by both
prokaryotic and eukaryotic cells might have a profound effect on experimental and clinical …

Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles

B György, TG Szabó, M Pásztói, Z Pál, P Misják… - Cellular and molecular …, 2011 - Springer
Release of membrane vesicles, a process conserved in both prokaryotes and eukaryotes,
represents an evolutionary link, and suggests essential functions of a dynamic extracellular …

Optical, electrochemical and electrical (nano) biosensors for detection of exosomes: A comprehensive overview

L Xu, N Shoaie, F Jahanpeyma, J Zhao… - Biosensors and …, 2020 - Elsevier
Exosomes are small extracellular vesicles involved in many physiological activities of cells
in the human body. Exosomes from cancer cells have great potential to be applied in clinical …

[HTML][HTML] Recent developments in the nomenclature, presence, isolation, detection and clinical impact of extracellular vesicles

E Van der Pol, AN Böing, EL Gool… - Journal of thrombosis and …, 2016 - Elsevier
The research field of extracellular vesicles (EVs), such as microparticles and exosomes, is
growing exponentially. The goal of this review is to provide an overview of recent …

Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer

V Pospichalova, J Svoboda, Z Dave… - Journal of …, 2015 - Taylor & Francis
Flow cytometry is a powerful method, which is widely used for high-throughput quantitative
and qualitative analysis of cells. However, its straightforward applicability for extracellular …