Polymerase chain reaction (PCR)-based methods: promising molecular tools in dentistry

S Shahi, SZ Vahed, N Fathi, S Sharifi - International journal of biological …, 2018 - Elsevier
Polymerase chain reaction (PCR) has become a popular diagnosis and research technique
in dentistry. Several studies show that high sensitivity and specificity of PCR method allows it …

Dental biofilm of symptomatic COVID‐19 patients harbours SARS‐CoV‐2

SC Gomes, S Fachin, JG da Fonseca… - Journal of clinical …, 2021 - Wiley Online Library
Abstract Aims SARS‐CoV‐2 RNA has been recovered from different sites in the human
body, including the mouth. The present study aimed to investigate the presence of SARS …

Knock-on effect of periodontitis to the pathogenesis of Alzheimer's disease?

F Leblhuber, J Huemer, K Steiner, JM Gostner… - Wiener Klinische …, 2020 - Springer
Background Alzheimer's disease has chronic inflammatory components, which can be
enhanced by systemic immune activation resulting in inflammation or vice versa. There is …

An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel

D Sun, D Xu, C Yang, MB Shahzad, Z Sun, J Xia… - Scientific Reports, 2016 - nature.com
In order to solve the challenging problem of microbial infections caused by microorganisms
on medical implants, it is imperative to develop novel antimicrobial biomaterials. This work …

[HTML][HTML] In Situ Anabolic Activity of Periodontal Pathogens Porphyromonas gingivalis and Filifactor alocis in Chronic Periodontitis

R Spooner, KM Weigel, PL Harrison, KL Lee… - Scientific reports, 2016 - nature.com
Porphyromonas gingivalis and Filifactor alocis are fastidious anaerobic bacteria strongly
associated with chronic forms of periodontitis. Our understanding of the growth activities of …

Clinical evaluation of a newly developed chairside test to determine periodontal pathogens

NB Arweiler, VK Marx, O Laugisch… - Journal of …, 2020 - Wiley Online Library
Background The subgingival microbiota as well as determination of markers such as
associated pathogens is still in the focus of dental research. The aim of this controlled …

Quantification by qPCR of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level

I Tomás, A Regueira-Iglesias, M López… - Frontiers in …, 2017 - frontiersin.org
Currently, there is little evidence available on the development of predictive models for the
diagnosis or prognosis of chronic periodontitis based on the qPCR quantification of …

A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics

J Kreuter, K Bica-Schröder, ÁM Pálvölgyi… - Analytical and …, 2024 - Springer
DNA-and RNA-based diagnostics play a pivotal role in accurately detecting and
characterizing health-relevant bacteria, offering insights into bacterial presence, viability and …

Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR—A Clinical Follow-Up Study

O Laugisch, TM Auschill, A Tumbrink, A Sculean… - Antibiotics, 2022 - mdpi.com
A chair-side test (CST) for five periodontal pathogens (Aggregatibacter
actinomycetemcomitans, Aa; Porphyromonas gingivalis, Pg; Prevotella intermedia, Pi; …

Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real‐time PCR and real‐time‐PCR …

M Sereti, A Zekeridou, J Cancela… - Clinical and …, 2021 - Wiley Online Library
Objectives The aim of the present methodological study was to evaluate the discrepancies in
the detection of a number of periodontally involved pathogenic bacteria obtained from …