Simulated microgravity: critical review on the use of random positioning machines for mammalian cell culture

SL Wuest, S Richard, S Kopp… - BioMed research …, 2015 - Wiley Online Library
Random Positioning Machines (RPMs) have been used since many years as a ground‐
based model to simulate microgravity. In this review we discuss several aspects of the RPM …

Modeling the impact of microgravity at the cellular level: implications for human disease

P Bradbury, H Wu, JU Choi, AE Rowan… - Frontiers in cell and …, 2020 - frontiersin.org
A lack of gravity experienced during space flight has been shown to have profound effects
on human physiology including muscle atrophy, reductions in bone density and immune …

Magnetic levitational bioassembly of 3D tissue construct in space

VA Parfenov, YD Khesuani, SV Petrov, PA Karalkin… - Science …, 2020 - science.org
Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a
rapidly emerging scaffold-and label-free approach and alternative conceptual advance in …

Growing tissues in real and simulated microgravity: new methods for tissue engineering

D Grimm, M Wehland, J Pietsch… - … Engineering Part B …, 2014 - liebertpub.com
Tissue engineering in simulated (s-) and real microgravity (r-μ g) is currently a topic in Space
medicine contributing to biomedical sciences and their applications on Earth. The principal …

The effects of weightlessness on the human organism and mammalian cells

J Pietsch, J Bauer, M Egli, M Infanger… - Current molecular …, 2011 - ingentaconnect.com
It has always been a desire of mankind to conquest Space. A major step in realizing this
dream was the completion of the International Space Station (ISS). Living there for several …

Tissue engineering under microgravity conditions–use of stem cells and specialized cells

D Grimm, M Egli, M Krüger, S Riwaldt… - Stem Cells and …, 2018 - liebertpub.com
Experimental cell research studying three-dimensional (3D) tissues in space and on Earth
using new techniques to simulate microgravity is currently a hot topic in Gravitational Biology …

The impact of simulated and real microgravity on bone cells and mesenchymal stem cells

C Ulbrich, M Wehland, J Pietsch… - BioMed research …, 2014 - Wiley Online Library
How microgravity affects the biology of human cells and the formation of 3D cell cultures in
real and simulated microgravity (r‐and s‐µg) is currently a hot topic in biomedicine. In r‐and …

Biomanufacturing of 3D tissue constructs in microgravity and their applications in human pathophysiological studies

Z Ren, AD Harriot, DB Mair, MK Chung… - Advanced …, 2023 - Wiley Online Library
The growing interest in bioengineering in‐vivo‐like 3D functional tissues has led to novel
approaches to the biomanufacturing process as well as expanded applications for these …

Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission

J Pietsch, X Ma, M Wehland, G Aleshcheva… - Biomaterials, 2013 - Elsevier
Human follicular thyroid cancer cells were cultured in Space to investigate the impact of
microgravity on 3D growth. For this purpose, we designed and constructed a cell container …

Mechanisms of three-dimensional growth of thyroid cells during long-term simulated microgravity

S Kopp, E Warnke, M Wehland, G Aleshcheva… - Scientific Reports, 2015 - nature.com
Three-dimensional multicellular spheroids (MCS) of human cells are important in cancer
research. We investigated possible mechanisms of MCS formation of thyroid cells. Both …