Polyethylene glycol and solutions of polyethylene glycol as green reaction media

J Chen, SK Spear, JG Huddleston, RD Rogers - Green Chemistry, 2005 - pubs.rsc.org
In this review, we examine the concept that aqueous biphasic reactive extraction (ABRE)
can successfully integrate the solvent properties of polyethylene glycol (PEG) and its phase …

Liquid–liquid phase separation in artificial cells

CD Crowe, CD Keating - Interface Focus, 2018 - royalsocietypublishing.org
Liquid–liquid phase separation (LLPS) in biology is a recently appreciated means of
intracellular compartmentalization. Because the mechanisms driving phase separations are …

Aqueous two-phase systems: A general overview

R Hatti-Kaul - Molecular biotechnology, 2001 - Springer
Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be
used for separation of cells, membranes, viruses, proteins, nucleic acids, and other …

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

AG Waghmare, MK Salve, JG LeBlanc… - Bioresources and …, 2016 - Springer
Background Many methods are available for the concentration of proteins; however, most
are not easily scalable due to costs, the need of specialized instruments and skilled workers …

Practical application of aqueous two-phase partition to process development for the recovery of biological products

M Rito-Palomares - Journal of Chromatography B, 2004 - Elsevier
The practical application of aqueous two-phase systems (ATPS) to process development
has been exploited for several years for the recovery of biological products. Unfortunately …

Micelle-mediated extraction as a tool for separation and preconcentration in metal analysis

CD Stalikas - TrAC Trends in Analytical Chemistry, 2002 - Elsevier
Micelle-based techniques can be utilised for the preconcentration and separation of metal
ions, which are either in their native form or in chelates that are produced after reaction …

Aqueous two-phase systems: a viable platform in the manufacturing of biopharmaceuticals

PAJ Rosa, IF Ferreira, AM Azevedo… - … of Chromatography A, 2010 - Elsevier
The number of biotechnology-based pharmaceuticals in the late-stage pipeline has been
increasing more than ever. As a result, there is an enhanced demand for more efficient and …

Design of environmentally friendly ionic liquid aqueous two-phase systems for the efficient and high activity extraction of proteins

Z Li, X Liu, Y Pei, J Wang, M He - Green Chemistry, 2012 - pubs.rsc.org
Ionic liquids (ILs) have numerous applications in industrial processes as a benign
alternative to conventional volatile organic solvents. However, many of them are toxic to …

Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing

AM Azevedo, PAJ Rosa, IF Ferreira… - Trends in …, 2009 - cell.com
The therapeutic use of proteins has created an increasing demand for feasible and
economical methods for both up-and downstream processes. However, whereas upstream …

Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

PG Mazzola, AM Lopes, FA Hasmann… - Journal of Chemical …, 2008 - Wiley Online Library
Two‐phase systems can be exploited in separation science for the extraction/purification of
desired biomolecules. This article reviews recent experimental work on the use of aqueous …