In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …

Conspectus Cellular structural biology methods are needed to characterize biological
processes at atomic resolution in the physiological environment of the cell. Toward this goal …

Abstract The Cost Action “Innovation with glycans: new frontiers from synthesis to new
biological targets”(INNOGLY) hosted the Workshop “Neuroglycoproteins in health and …

Deuterated biomolecules such as proteins, lipids, and DNA are widely used in neutron
scattering experiments. This is due to the unique scattering properties of 2 H, including a …

Although very effective in decreasing NMR relaxation of large proteins, homogeneous
deuteration can be costly, and anyway unsuitable for recombinant production in metazoan …

Stable isotope enrichment in proteins is necessary for high-resolution nuclear magnetic
resonance (NMR) experiments. Although methods for 13 C, 15 N and 2 H-enrichment in …