Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

MT Parker, K Knop, AV Sherwood, NJ Schurch… - Elife, 2020 - elifesciences.org
Understanding genome organization and gene regulation requires insight into RNA
transcription, processing and modification. We adapted nanopore direct RNA sequencing to …

The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops

X Cheng, Q He, S Tang, H Wang, X Zhang… - New …, 2021 - Wiley Online Library
Salt stress triggers the overdose accumulation of reactive oxygen species (ROS) in crop
plants, leading to severe oxidative damage to living tissues. MicroRNAs (miRNAs) act as …

The YTHDF proteins ECT2 and ECT3 bind largely overlapping target sets and influence target mRNA abundance, not alternative polyadenylation

L Arribas-Hernández, S Rennie, M Schon, C Porcelli… - Elife, 2021 - elifesciences.org
Gene regulation via N6-methyladenosine (m6A) in mRNA involves RNA-binding proteins
that recognize m6A via a YT521-B homology (YTH) domain. The plant YTH domain proteins …

Global approaches for profiling transcription initiation

RA Policastro, GE Zentner - Cell Reports Methods, 2021 - cell.com
Transcription start site (TSS) selection influences transcript stability and translation as well
as protein sequence. Alternative TSS usage is pervasive in organismal development, is a …

Epigenetic reprogramming rewires transcription during the alternation of generations in Arabidopsis

M Borg, RK Papareddy, R Dombey, E Axelsson… - Elife, 2021 - elifesciences.org
Alternation between morphologically distinct haploid and diploid life forms is a defining
feature of most plant and algal life cycles, yet the underlying molecular mechanisms that …

[HTML][HTML] Enjoy the silence: Canonical and non-canonical RNA silencing activity during plant sexual reproduction

J Cheng, G Martinez - Current Opinion in Plant Biology, 2024 - Elsevier
Plants produce small RNAs that accomplish a surprisingly versatile number of functions. The
heterogeneity of functions of plant small RNAs is evident at the tissue-specific level. In …

Chromatin regulates expression of small RNAs to help maintain transposon methylome homeostasis in Arabidopsis

RK Papareddy, K Páldi, S Paulraj, P Kao, S Lutzmayer… - Genome Biology, 2020 - Springer
Background Eukaryotic genomes are partitioned into euchromatic and heterochromatic
domains to regulate gene expression and other fundamental cellular processes. However …

The embryonic transcriptome of Arabidopsis thaliana

F Hofmann, MA Schon, MD Nodine - Plant reproduction, 2019 - Springer
Key message Arabidopsis embryos possess unique transcriptomes relative to other plant
tissues including somatic embryos, and can be partitioned into four transcriptional phases …

Causal role of a promoter polymorphism in natural variation of the Arabidopsis floral repressor gene FLC

P Zhu, M Schon, J Questa, M Nodine, C Dean - Current Biology, 2023 - cell.com
Noncoding polymorphism frequently associates with phenotypic variation, but causation and
mechanism are rarely established. Noncoding single-nucleotide polymorphisms (SNPs) …

Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

MT Parker, K Knop, V Zacharaki, AV Sherwood… - Elife, 2021 - elifesciences.org
Genes involved in disease resistance are some of the fastest evolving and most diverse
components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) …