Improving microalgae for biotechnology—From genetics to synthetic biology–Moving forward but not there yet

V Kselíková, A Singh, V Bialevich, M Čížková… - Biotechnology …, 2022 - Elsevier
Microalgae are a diverse group of photosynthetic organisms that can be exploited for the
production of different compounds, ranging from crude biomass and biofuels to high value …

Methodological review of genetic engineering approaches for non-model algae

M Mosey, D Douchi, EP Knoshaug, LML Laurens - Algal Research, 2021 - Elsevier
Despite the tremendous potential of algae to contribute to a future bioeconomy, there are
practical and theoretical limitations to how well naturally sourced species can perform in an …

Genetic mechanisms underlying increased microalgal thermotolerance, maximal growth rate, and yield on light following adaptive laboratory evolution

R Barten, DJM Van Workum, E de Bakker, J Risse… - BMC biology, 2022 - Springer
Background Adaptive laboratory evolution (ALE) is a powerful method for strain optimization
towards abiotic stress factors and for identifying adaptation mechanisms. In this study, the …

Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity

A Krishnan, M Cano, DA Karns, TA Burch… - Plant …, 2023 - Wiley Online Library
High cellular pigment levels in dense microalgal cultures contribute to excess light
absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri …

Picochlorum celeri as a model system for robust outdoor algal growth in seawater

A Krishnan, M Likhogrud, M Cano, S Edmundson… - Scientific reports, 2021 - nature.com
With fast growth rates, broad halotolerance and the ability to thrive at high temperatures,
algae in the genus Picochlorum are emerging as promising biomass producers. Recently …

Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light

S Steichen, A Deshpande, M Mosey, J Loob… - Nature …, 2024 - nature.com
Carbon capture and biochemical storage are some of the primary drivers of photosynthetic
yield and productivity. To elucidate the mechanisms governing carbon allocation, we …

[HTML][HTML] The synthetic future of algal genomes

HD Goold, JL Moseley, KJ Lauersen - Cell Genomics, 2024 - cell.com
Algae are diverse organisms with significant biotechnological potential for resource
circularity. Taking inspiration from fermentative microbes, engineering algal genomes holds …

Small cells with big photosynthetic productivities: biotechnological potential of the Picochlorum genus

A Krishnan, LR Dahlin, MT Guarnieri… - Trends in …, 2024 - cell.com
The Picochlorum genus is a distinctive eukaryotic green-algal clade that is the focus of
several current biotechnological studies. It is capable of extremely rapid growth rates and …

Development of the high-productivity marine microalga, Picochlorum renovo, as a photosynthetic protein secretion platform

LR Dahlin, MT Guarnieri - Algal Research, 2021 - Elsevier
Microalgae are remarkable in their ability to convert CO 2 and sunlight into renewable
biomass and bioproducts. Here, we have established a novel photosynthetic protein …

[HTML][HTML] Proximate biomass characterization of the high productivity marine microalga Picochlorum celeri TG2

AJ LaPanse, A Krishnan, G Dennis, DAJ Karns… - Plant Physiology and …, 2024 - Elsevier
Microalgae are compelling renewable resources with applications including biofuels,
bioplastics, nutrient supplements, and cosmetic products. Picochlorum celeri is an alga with …