Approaches to functional genomics in filamentous fungi

RJ Weld, KM Plummer, MA Carpenter, HJ Ridgway - Cell research, 2006 - nature.com
The study of gene function in filamentous fungi is a field of research that has made great
advances in very recent years. A number of transformation and gene manipulation strategies …

Advances in genetically engineered (transgenic) plants in pest management—an over view

RM Babu, A Sajeena, K Seetharaman, MS Reddy - Crop protection, 2003 - Elsevier
Transgenic plants are produced via Agrobacterium mediated transformation and other direct
DNA transfer methods. A number of transgenes conferring resistance to insects, diseases …

In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA

MLP Tinoco, BBA Dias, RC Dall'Astta, JA Pamphile… - BMC biology, 2010 - Springer
Background Self-complementary RNA transcripts form a double-stranded RNA (dsRNA) that
triggers a sequence-specific mRNA degradation, in a process known as RNA interference …

Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana

SH Ying, MG Feng - Applied microbiology and biotechnology, 2006 - Springer
A novel system was developed for efficient transformation of the fungal biocontrol agent
Beauveria bassiana. Competent blastospores were prepared and stored in LiAc-and …

Agrobacterium-mediated insertional mutagenesis (AIM) of the entomopathogenic fungus Beauveria bassiana

A Leclerque, H Wan, A Abschütz, S Chen, GV Mitina… - Current Genetics, 2004 - Springer
Agrobacterium tumefaciens was used to stably transform the entomopathogenic
deuteromycete Beauveria bassiana to hygromycin B resistance by integration of the hph …

[图书][B] Handbook of fungal biotechnology

DK Arora - 2003 - taylorfrancis.com
The Handbook of Fungal Biotechnology offers the newest developments from the frontiers of
fungal biochemical and molecular processes and industrial and semi-industrial applications …

The Ifchit1 chitinase gene acts as a critical virulence factor in the insect pathogenic fungus Isaria fumosorosea

Z Huang, Y Hao, T Gao, Y Huang, S Ren… - Applied microbiology …, 2016 - Springer
The filamentous fungus, Isaria fumosorosea, is a promising insect biological control agent.
Chitinases have been implicated in targeting insect cuticle structures, with biotechnological …

Stable transformation of the oomycete, Phytophthora infestans, using microprojectile bombardment

C Cvitanich, HS Judelson - Current genetics, 2003 - Springer
Germinated asexual sporangia, zoospores, and mycelia of Phytophthora infestans were
transformed to G418-resistance by microprojectile bombardment. After optimization, an …

[HTML][HTML] Genetic manipulation of Fonsecaea pedrosoi using particles bombardment and Agrobacterium mediated transformation

CS Florencio, FAS Brandão, M de Mello Teixeira… - Microbiological …, 2018 - Elsevier
Fonsecaea pedrosoi, a melanized fungal pathogen that causes Chromoblastomycosis, a
human disease with a worldwide distribution. Biolistic is a widely used technique for direct …

Transformation of the entomopathogenic fungus Paecilomyces fumosoroseus with Agrobacterium tumefaciens

IGP Lima, RTD Duarte, L Furlaneto… - Letters in applied …, 2006 - academic.oup.com
Aims: To test the suitability of the Agrobacterium tumefaciens‐mediated transformation
(AMT) method with Paecilomyces fumosoroseus, a fungal pathogen that causes diseases in …