The pausing zone and control of RNA polymerase II elongation by Spt5: Implications for the pause-release model
The pause-release model of transcription proposes that 40–100 bases from the start site
RNA Pol II pauses, followed by release into productive elongation. Pause release is …
RNA Pol II pauses, followed by release into productive elongation. Pause release is …
Global approaches for profiling transcription initiation
RA Policastro, GE Zentner - Cell Reports Methods, 2021 - cell.com
Transcription start site (TSS) selection influences transcript stability and translation as well
as protein sequence. Alternative TSS usage is pervasive in organismal development, is a …
as protein sequence. Alternative TSS usage is pervasive in organismal development, is a …
Nucleotide-level linkage of transcriptional elongation and polyadenylation
JV Geisberg, Z Moqtaderi, N Fong, B Erickson… - Elife, 2022 - elifesciences.org
Alternative polyadenylation yields many mRNA isoforms whose 3'termini occur
disproportionately in clusters within 3'untranslated regions. Previously, we showed that …
disproportionately in clusters within 3'untranslated regions. Previously, we showed that …
The EDC4‐XRN1 interaction controls P‐body dynamics to link mRNA decapping with decay
WR Brothers, F Ali, S Kajjo, MR Fabian - The EMBO Journal, 2023 - embopress.org
Deadenylation‐dependent mRNA decapping and decay is the major cytoplasmic mRNA
turnover pathway in eukaryotes. Many mRNA decapping and decay factors are associated …
turnover pathway in eukaryotes. Many mRNA decapping and decay factors are associated …
A convenient strategy to clone small RNA and mRNA for high-throughput sequencing
High-throughput sequencing has become a standard tool for analyzing RNA and DNA. This
method usually needs a cDNA/DNA library ligated with specific 5′ and 3′ linkers. Unlike …
method usually needs a cDNA/DNA library ligated with specific 5′ and 3′ linkers. Unlike …
Solid-phase XRN1 reactions for RNA cleavage: application in single-molecule sequencing
US Athapattu, CA Amarasekara, JR Immel… - Nucleic acids …, 2021 - academic.oup.com
Modifications in RNA are numerous (∼ 170) and in higher numbers compared to DNA (∼ 5)
making the ability to sequence an RNA molecule to identify these modifications highly …
making the ability to sequence an RNA molecule to identify these modifications highly …
Nanopore sequencing of internal 2′-PO4 modifications installed by RNA repair
LK White, SM Strugar, A MacFadden, JR Hesselberth - RNA, 2023 - rnajournal.cshlp.org
Ligation by plant and fungal RNA ligases yields an internal 2′-phosphate group on each
RNA ligation product. In budding yeast, this covalent mark occurs at the splice junction of …
RNA ligation product. In budding yeast, this covalent mark occurs at the splice junction of …
Direct detection of RNA repair by nanopore sequencing
LK White, SM Strugar, A MacFadden, JR Hesselberth - bioRxiv, 2022 - biorxiv.org
Ligation by plant and fungal RNA ligases yields an internal 2′-phosphate group on each
RNA ligation product. In budding yeast, this covalent mark occurs at the splice junction of …
RNA ligation product. In budding yeast, this covalent mark occurs at the splice junction of …
[HTML][HTML] Strategies and best practice in cloning small RNAs
High-throughput sequencing has become a standard and powerful tool for analyzing nucleic
acids primarily due to its sensitivity and convenience. Small RNAs play important roles in …
acids primarily due to its sensitivity and convenience. Small RNAs play important roles in …
[图书][B] Investigating how processing body ribonucleoprotein granules regulate mRNA decay in human cells
WR Brothers - 2023 - search.proquest.com
The controlled degradation of mRNAs plays a critical role in post-transcriptionally regulating
gene expression. In the cytoplasm, most mRNA decay occurs through a deadenylation …
gene expression. In the cytoplasm, most mRNA decay occurs through a deadenylation …