Highlights•Isoprenoid precursors and CoA are essential metabolites generated by
apicoplast-localized enzymes.•Isoprenoids play an essential role in apicoplast …

Apicomplexan parasites encompass diverse pathogens for humans and animals, including
the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma …

Malaria parasites uniquely depend on protein secretion for their obligate intracellular
lifestyle but approaches for dissecting Plasmodium-secreted protein functions are limited …

Malaria parasites contain an essential organelle called the apicoplast that houses metabolic
pathways for fatty acid, heme, isoprenoid, and iron–sulfur cluster synthesis. Surprisingly …

One of the most powerful approaches to understanding gene function involves turning
genes on and off at will and measuring the impact at the cellular or organismal level. This …

Malaria parasites rely on a plastid organelle for survival during the blood stages of infection.
However, the entire organelle is dispensable as long as the isoprenoid precursor …

In the glucose-rich milieu of red blood cells, asexually replicating malarial parasites mainly
rely on glycolysis for ATP production, with limited carbon flux through the mitochondrial …

Iron-sulfur clusters (FeS) are ancient and ubiquitous protein cofactors that play fundamental
roles in many aspects of cell biology. These cofactors cannot be scavenged or trafficked …

Ferredoxin (Fd) and ferredoxin-NADP+ reductase (FNR) form a redox system that is
hypothesized to play a central role in the maintenance and function of the apicoplast …

Malaria, caused by infection with Plasmodium parasites, remains a significant global health
concern. For decades, genetic intractability and limited tools hindered our ability to study …