A comprehensive summary of a decade development of the recombinase polymerase amplification

J Li, J Macdonald, F Von Stetten - Analyst, 2019 - pubs.rsc.org
Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …

Recombinase polymerase amplification: Basics, applications and recent advances

IM Lobato, CK O'Sullivan - Trac Trends in analytical chemistry, 2018 - Elsevier
Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …

Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

K Kant, MA Shahbazi, VP Dave, TA Ngo… - Biotechnology …, 2018 - Elsevier
Rapid detection of foodborne pathogens at an early stage is imperative for preventing the
outbreak of foodborne diseases, known as serious threats to human health. Conventional …

Point-of-care-testing of nucleic acids by microfluidics

L Zhang, B Ding, Q Chen, Q Feng, L Lin… - TrAC Trends in Analytical …, 2017 - Elsevier
Nucleic acid detection offers critical information in diagnosing pathogen infection and
monitoring disease treatment. Point-of-care testing (POCT), with ideal characteristics such …

Assessment of bacterial viability: a comprehensive review on recent advances and challenges

SS Kumar, AR Ghosh - Microbiology, 2019 - microbiologyresearch.org
Assessing bacterial contamination in environmental samples is critical in determining threats
to public health. The classical methods are time-consuming and only recognize species that …

Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid

D Gao, X Guo, Y Yang, H Shi, R Hao, S Wang… - Journal of Biological …, 2022 - Springer
The frequency of outbreaks of newly emerging infectious diseases has increased in recent
years. The coronavirus disease 2019 (COVID-19) outbreak in late 2019 has caused a global …

Recombinase polymerase amplification integrated with microfluidics for nucleic acid testing at point of care

Y Bai, J Ji, F Ji, S Wu, Y Tian, B Jin, Z Li - Talanta, 2022 - Elsevier
Nucleic acid testing (NAT) implemented on a portable, miniaturized, and integrated device
with rapid and sensitive results readout is highly demanded for pathogen detection or …

Current and future perspectives on isothermal nucleic acid amplification technologies for diagnosing infections

GA Obande, KK Banga Singh - Infection and drug resistance, 2020 - Taylor & Francis
Nucleic acid amplification technology (NAAT) has assumed a critical position in disease
diagnosis in recent times and contributed significantly to healthcare. Application of these …

Bacterial classification and antibiotic susceptibility testing on an integrated microfluidic platform

AA Sklavounos, CR Nemr, SO Kelley, AR Wheeler - Lab on a Chip, 2021 - pubs.rsc.org
With the prevalence of bacterial infections and increasing levels of antibiotic resistance
comes the need for rapid and accurate methods for bacterial classification (BC) and …

Multiplexed isothermal nucleic acid amplification

O Mayboroda, I Katakis, CK O'Sullivan - Analytical biochemistry, 2018 - Elsevier
Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …