Development of lignocellulosic biorefineries for the sustainable production of biofuels: towards circular bioeconomy

A Yadav, V Sharma, ML Tsai, CW Chen, PP Sun… - Bioresource …, 2023 - Elsevier
The idea of environment friendly and affordable renewable energy resources has prompted
the industry to focus on the set up of biorefineries for sustainable bioeconomy …

Design of novel enzyme biocatalysts for industrial bioprocess: Harnessing the power of protein engineering, high throughput screening and synthetic biology

A Madhavan, KB Arun, P Binod, R Sirohi… - Bioresource …, 2021 - Elsevier
Biocatalysts have wider applications in various industries. Biocatalysts are generating
bigger attention among researchers due to their unique catalytic properties like activity …

A consolidated review of commercial-scale high-value products from lignocellulosic biomass

B Zheng, S Yu, Z Chen, YX Huo - Frontiers in Microbiology, 2022 - frontiersin.org
For decades, lignocellulosic biomass has been introduced to the public as the most
important raw material for the environmentally and economically sustainable production of …

Progress in ameliorating beneficial characteristics of microbial cellulases by genetic engineering approaches for cellulose saccharification

A Dadwal, S Sharma, T Satyanarayana - Frontiers in microbiology, 2020 - frontiersin.org
Lignocellulosic biomass is a renewable and sustainable energy source. Cellulases are the
enzymes that cleave β-1, 4-glycosidic linkages in cellulose to liberate sugars that can be …

Molecular biology interventions for activity improvement and production of industrial enzymes

SK Bhatia, N Vivek, V Kumar, N Chandel, M Thakur… - Bioresource …, 2021 - Elsevier
Metagenomics and directed evolution technology have brought a revolution in search of
novel enzymes from extreme environment and improvement of existing enzymes and tuning …

Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1

EF El-Khamisi, EAM Soliman, GM El-Sayed… - Microbial Cell …, 2024 - Springer
Background In this study, we isolated a cellulase-producing bacterium, Bacillus
amyloliquefaciens strain elh, from rice peel. We employed two optimization methods to …

Immobilization and Characterization of a Processive Endoglucanase EG5C-1 from Bacillus subtilis on Melamine–Glutaraldehyde Dendrimer-Functionalized Magnetic …

X Li, J Chen, B Wu, Z Gao, B He - Nanomaterials, 2024 - mdpi.com
Exploring an appropriate immobilization approach to enhance catalytic activity and
reusability of cellulase is of great importance to reduce the price of enzymes and promote …

Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant …

PV Gavande, P Nath, K Kumar, N Ahmed… - International Journal of …, 2022 - Elsevier
Abstract Gene encoding endoglucanase, RfGH5_4 from R. flavefaciens FD-1 v3 was cloned,
expressed in Escherichia coli BL21 (DE3) cells and purified. RfGH5_4 showed molecular …

Harnessing the Keratinolytic Activity of Bacillus licheniformis Through Random Mutagenesis Using Ultraviolet and Laser Irradiations

JA Tuly, H Ma, HM Zabed, Y Dong, Q Janet… - Applied Biochemistry …, 2021 - Springer
Keratinase is one of the important proteases, which is widely used for converting keratin of
the keratinaceous materials into various value-added products. In this study, a popular …

Multifunctionality and mechanism of processivity of family GH5 endoglucanase, RfGH5_4 from Ruminococcus flavefaciens on lignocellulosic polymers

PV Gavande, K Kumar, J Ahmed, A Goyal - International Journal of …, 2023 - Elsevier
Abstract Multifunctional endoglucanase, RfGH5_4 from Ruminococcus flavefaciens showed
(β/α) 8-TIM barrel structure by homology modeling. Glu168 and Glu292 residues acted as …