Techniques for the analysis of protein-protein interactions in vivo

S Xing, N Wallmeroth, KW Berendzen… - Plant physiology, 2016 - academic.oup.com
Identifying key players and their interactions is fundamental for understanding biochemical
mechanisms at the molecular level. The ever-increasing number of alternative ways to …

Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions

MM Goodin, D Zaitlin, RA Naidu… - Molecular plant …, 2008 - Am Phytopath Society
Nicotiana benthamiana is the most widely used experimental host in plant virology, due
mainly to the large number of diverse plant viruses that can successfully infect it …

Modulating plant growth–metabolism coordination for sustainable agriculture

S Li, Y Tian, K Wu, Y Ye, J Yu, J Zhang, Q Liu, M Hu… - Nature, 2018 - nature.com
Enhancing global food security by increasing the productivity of green revolution varieties of
cereals risks increasing the collateral environmental damage produced by inorganic …

The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality

S Wang, S Li, Q Liu, K Wu, J Zhang, S Wang, Y Wang… - Nature …, 2015 - nature.com
The deployment of heterosis in the form of hybrid rice varieties has boosted grain yield, but
grain quality improvement still remains a challenge. Here we show that a quantitative trait …

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

FH Wu, SC Shen, LY Lee, SH Lee, MT Chan, CS Lin - Plant methods, 2009 - Springer
Background Protoplasts isolated from leaves are useful materials in plant research. One
application, the transient expression of recombinant genes using Arabidopsis mesophyll …

[HTML][HTML] Firefly luciferase complementation imaging assay for protein-protein interactions in plants

H Chen, Y Zou, Y Shang, H Lin, Y Wang, R Cai… - Plant …, 2008 - ncbi.nlm.nih.gov
The development of sensitive and versatile techniques to detect protein-protein interactions
in vivo is important for understanding protein functions. The previously described …

A ubiquitin‐10 promoter‐based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression …

C Grefen, N Donald, K Hashimoto, J Kudla… - The Plant …, 2010 - Wiley Online Library
Fluorescent tagging of proteins and confocal imaging techniques have become methods of
choice in analysing the distributions and dynamic characteristics of proteins at the …

Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta

R Waadt, LK Schmidt, M Lohse, K Hashimoto… - The Plant …, 2008 - Wiley Online Library
The specificity of intracellular signaling and developmental patterning in biological systems
relies on selective interactions between different proteins in specific cellular compartments …

The NB‐LRR gene Pm60 confers powdery mildew resistance in wheat

S Zou, H Wang, Y Li, Z Kong, D Tang - New Phytologist, 2018 - Wiley Online Library
Powdery mildew is one of the most devastating diseases of wheat. To date, few powdery
mildew resistance genes have been cloned from wheat due to the size and complexity of the …

Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells

TK Kerppola - Annu. Rev. Biophys., 2008 - annualreviews.org
Protein interactions are a fundamental mechanism for the generation of biological regulatory
specificity. The study of protein interactions in living cells is of particular significance …