Near-infrared fluorescent proteins, biosensors, and optogenetic tools engineered from phytochromes

KG Chernov, TA Redchuk, ES Omelina… - Chemical …, 2017 - ACS Publications
Phytochrome photoreceptors absorb far-red and near-infrared (NIR) light and regulate light
responses in plants, fungi, and bacteria. Their multidomain structure and autocatalytic …

Diverse light responses of cyanobacteria mediated by phytochrome superfamily photoreceptors

LB Wiltbank, DM Kehoe - Nature Reviews Microbiology, 2019 - nature.com
Cyanobacteria are an evolutionarily and ecologically important group of prokaryotes. They
exist in diverse habitats, ranging from hot springs and deserts to glaciers and the open …

Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

OS Oliinyk, AA Shemetov, S Pletnev… - Nature …, 2019 - nature.com
From a single domain of cyanobacteriochrome (CBCR) we developed a near-infrared (NIR)
fluorescent protein (FP), termed miRFP670nano, with excitation at 645 nm and emission at …

Cyanobacteriochromes: photoreceptors covering the entire UV-to-visible spectrum

K Fushimi, R Narikawa - Current opinion in structural biology, 2019 - Elsevier
Highlights•Combination of four color-tuning mechanisms results in diverse spectral
properties.•Structural information uncovers general and specific photoconversion …

Phytochrome evolution in 3D: deletion, duplication, and diversification

NC Rockwell, JC Lagarias - New Phytologist, 2020 - Wiley Online Library
Canonical plant phytochromes are master regulators of photomorphogenesis and the shade
avoidance response. They are also part of a widespread superfamily of photoreceptors with …

Chromatic acclimation in cyanobacteria: a diverse and widespread process for optimizing photosynthesis

JE Sanfilippo, L Garczarek, F Partensky… - Annual review of …, 2019 - annualreviews.org
Chromatic acclimation (CA) encompasses a diverse set of molecular processes that involve
the ability of cyanobacterial cells to sense ambient light colors and use this information to …

The red edge: bilin-binding photoreceptors as optogenetic tools and fluorescence reporters

K Tang, HM Beyer, MD Zurbriggen… - Chemical …, 2021 - ACS Publications
This review adds the bilin-binding phytochromes to the Chemical Reviews thematic issue
“Optogenetics and Photopharmacology”. The work is structured into two parts. We first …

Photoreceptor engineering

T Ziegler, A Möglich - Frontiers in molecular biosciences, 2015 - frontiersin.org
Sensory photoreceptors not only control diverse adaptive responses in Nature, but as light-
regulated actuators they also provide the foundation for optogenetics, the non-invasive and …

Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives

R Ohlendorf, A Möglich - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Numerous photoreceptors and genetic circuits emerged over the past two decades and now
enable the light-dependent ie, optogenetic, regulation of gene expression in bacteria …

Picocyanobacteria and deep-ocean fluorescent dissolved organic matter share similar optical properties

Z Zhao, M Gonsior, J Luek, S Timko, H Ianiri… - Nature …, 2017 - nature.com
Marine chromophoric dissolved organic matter (CDOM) and its related fluorescent
components (FDOM), which are widely distributed but highly photobleached in the surface …