Process intensification in biologics manufacturing
J Strube, R Ditz, M Kornecki, M Huter, A Schmidt… - … and Processing-Process …, 2018 - Elsevier
Over the last decade, the term process intensification (PI) has been applied almost
exclusively to large scale chemical processes, but hardly to aspects of biopharmaceutical …
exclusively to large scale chemical processes, but hardly to aspects of biopharmaceutical …
Molecular simulation of adsorption and its implications to protein chromatography: A review
L Zhang, Y Sun - Biochemical Engineering Journal, 2010 - Elsevier
Adsorption on solid surface is of fundamental importance in the research and development
of chromatographic science and technology. Due to the limitation of the currently available …
of chromatographic science and technology. Due to the limitation of the currently available …
Biomimetic design of affinity peptide ligands for human IgG based on protein A-IgG complex
WW Zhao, FF Liu, QH Shi, XY Dong, Y Sun - Biochemical engineering …, 2014 - Elsevier
Staphylococcal protein A (SpA) has been widely used as an affinity ligand for the purification
of immunoglobulin G (IgG). Based on the affinity motif of SpA, we have herein developed a …
of immunoglobulin G (IgG). Based on the affinity motif of SpA, we have herein developed a …
Molecular insight into the ligand–IgG interactions for 4-mercaptoethyl-pyridine based hydrophobic charge-induction chromatography
DQ Lin, HF Tong, HY Wang, SJ Yao - The Journal of Physical …, 2012 - ACS Publications
Hydrophobic charge-induction chromatography (HCIC) with 4-mercaptoethyl-pyridine (MEP)
as the ligand is a novel technology for antibody purification. In the present work, the …
as the ligand is a novel technology for antibody purification. In the present work, the …
Molecular modeling of Protein A affinity chromatography
The properties of the complex between fragment B of Protein A and the Fc domain of IgG
were investigated adopting molecular dynamics with the intent of providing useful insight …
were investigated adopting molecular dynamics with the intent of providing useful insight …
Structural characterization of a Protein A mimetic peptide dendrimer bound to human IgG
Understanding the chemical physical properties of protein binding sites is at the basis of the
rational design of protein ligands. The hinge region of the Fc fragment of immunoglobulin G …
rational design of protein ligands. The hinge region of the Fc fragment of immunoglobulin G …
Machine Learning Models for Predicting Monoclonal Antibody Biophysical Properties from Molecular Dynamics Simulations and Deep Learning-Based Surface …
Monoclonal antibodies (mAbs) have found extensive applications and development in
treating various diseases. From the pharmaceutical industry's perspective, the journey from …
treating various diseases. From the pharmaceutical industry's perspective, the journey from …
High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization
M Kangwa, V Yelemane, AN Polat, KDD Gorrepati… - AMB Express, 2015 - Springer
The major platform for high level recombinant protein production is based on genetically
modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to …
modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to …
Understanding the molecular recognition between antibody fragments and protein A biomimetic ligand
Affinity chromatography with protein A from Staphylococcus aureus (SpA) is the most
widespread and accepted methodology for antibody capture during the downstream process …
widespread and accepted methodology for antibody capture during the downstream process …
Effects of ligand density on hydrophobic charge induction chromatography: molecular dynamics simulation
L Zhang, G Zhao, Y Sun - The Journal of Physical Chemistry B, 2010 - ACS Publications
High ligand density is usually required in hydrophobic charge induction chromatography
(HCIC) for high adsorption capacity. However, it is not clear to what extent the ligand density …
(HCIC) for high adsorption capacity. However, it is not clear to what extent the ligand density …