RNA-based therapeutics have shown great promise in treating a broad spectrum of
diseases through various mechanisms including knockdown of pathological genes …

Gene therapy has gained significant attention over the past two decades as a potential
method for treating genetic disorders such as severe combined immunodeficiency, 1 cystic …

Lipid nanoparticles (LNPs) are the most clinically advanced delivery vehicles for RNA and
have enabled the development of RNA-based drugs such as the mRNA COVID-19 vaccines …

A key end goal of gene delivery research is to develop clinically relevant vectors that can be
used to combat elusive diseases such as AIDS. Despite promising engineering strategies …

CRISPR/Cas9 genome editing platforms are widely applied as powerful tools in basic
research and potential therapeutics for genome regulation. The appropriate alternative of …

Cationic lipids are one of the most widely used nonviral vectors for gene delivery and are
especially attractive because they can be easily synthesized and extensively characterized …

Cationic lipids have become known as one of the most versatile tools for the delivery of
DNA, RNA and many other therapeutic molecules, and are especially attractive because …

Cationic lipid-mediated gene transfer is widely used for their advantages over viral gene
transfer because it is non-immunogenic, easy to produce and not oncogenic. The main …

As effective non-viral vectors of gene therapy, cationic lipids still have the problem of toxicity,
which has become one of the main bottlenecks for their applications. The toxicity of cationic …

The structure of cationic lipids is a major factor for their transfection activity. A cationic lipid
generally contains four functional domains: a hydrophilic headgroup, a linker, a backbone …