Brillouin microscopy: an emerging tool for mechanobiology

R Prevedel, A Diz-Muñoz, G Ruocco, G Antonacci - Nature methods, 2019 - nature.com
The role and importance of mechanical properties of cells and tissues in cellular function,
development and disease has widely been acknowledged, however standard techniques …

Recent progress and current opinions in Brillouin microscopy for life science applications

G Antonacci, T Beck, A Bilenca, J Czarske… - Biophysical …, 2020 - Springer
Many important biological functions and processes are reflected in cell and tissue
mechanical properties such as elasticity and viscosity. However, current techniques used for …

Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells

H Keshmiri, D Cikes, M Samalova, L Schindler… - Nature …, 2024 - nature.com
Maintaining and modulating mechanical anisotropy is essential for biological processes.
However, how this is achieved at the microscopic scale in living soft matter is not always …

Pulsed stimulated Brillouin microscopy enables high-sensitivity mechanical imaging of live and fragile biological specimens

F Yang, C Bevilacqua, S Hambura, A Neves… - Nature …, 2023 - nature.com
Brillouin microscopy is an emerging optical elastography technique capable of assessing
mechanical properties of biological samples in a three-dimensional, all-optical and …

Tumor cell nuclei soften during transendothelial migration

AB Roberts, J Zhang, VR Singh, M Nikolić… - Journal of …, 2021 - Elsevier
During cancer metastasis, tumor cells undergo significant deformation in order to traverse
through endothelial cell junctions in the walls of blood vessels. As cells pass through narrow …

High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy

I Remer, R Shaashoua, N Shemesh, A Ben-Zvi… - Nature …, 2020 - nature.com
Label-free, non-contact imaging with mechanical contrast and optical sectioning is a
substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this …

High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

C Bevilacqua, JM Gomez, UM Fiuza, CJ Chan… - Nature …, 2023 - nature.com
Brillouin microscopy can assess mechanical properties of biological samples in a three-
dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead …

Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes

J Zhang, G Scarcelli - Nature protocols, 2021 - nature.com
Several techniques have been developed over the past few decades to assess the
mechanical properties of biological samples, which has fueled a rapid growth in the fields of …

Nuclear mechanics within intact cells is regulated by cytoskeletal network and internal nanostructures

J Zhang, F Alisafaei, M Nikolić, XA Nou, H Kim… - Small, 2020 - Wiley Online Library
The mechanical properties of the cellular nucleus are extensively studied as they play a
critical role in important processes, such as cell migration, gene transcription, and stem cell …

Correlative all-optical quantification of mass density and mechanics of subcellular compartments with fluorescence specificity

R Schlüßler, K Kim, M Nötzel, A Taubenberger… - Elife, 2022 - elifesciences.org
Quantitative measurements of physical parameters become increasingly important for
understanding biological processes. Brillouin microscopy (BM) has recently emerged as one …