Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

Spatial omics technologies enable a deeper understanding of cellular organizations and
interactions within a tissue of interest. These assays can identify specific compartments or …

With the emergence of RNA sequencing (RNA-seq) technologies, RNA-based biomolecules
hold expanded promise for their diagnostic, prognostic and therapeutic applicability in …

As the use of RNA-seq has popularized, there is an increasing consciousness of the
importance of experimental design, bias removal, accurate quantification and control of false …

Assessing the reproducibility, accuracy and utility of massively parallel DNA sequencing
platforms remains an ongoing challenge. Here the Association of Biomolecular Resource …

Background As large-scale studies of gene expression with multiple sources of biological
and technical variation become widely adopted, characterizing these drivers of variation …

Recent technical developments have enabled the transcriptomes of hundreds of cells to be
assayed in an unbiased manner, opening up the possibility that new subpopulations of cells …

Single-cell RNA-sequencing (scRNA-seq) has emerged as a revolutionary tool that allows
us to address scientific questions that eluded examination just a few years ago. With the …

We present primary results from the Sequencing Quality Control (SEQC) project,
coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life …

We present an in silico approach to identifying neoepitopes derived from intron retention
events in tumor transcriptomes. Using mass spectrometry immunopeptidome analysis, we …