The formation of oximes and hydrazones is employed in numerous scientific fields as a
simple and versatile conjugation strategy. This imine-forming reaction is applied in fields as …

In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …

Over the last decade, the ability to genetically encode unnatural amino acids (UAAs) has
evolved rapidly. The programmed incorporation of UAAs into recombinant proteins relies on …

Genetic code expansion and reprogramming enable the site-specific incorporation of
diverse designer amino acids into proteins produced in cells and animals. Recent advances …

Chemical modification of proteins is an important tool for probing natural systems, creating
therapeutic conjugates and generating novel protein constructs. Site-selective reactions …

The genetic incorporation of the 22nd proteinogenic amino acid, pyrrolysine (Pyl) at amber
codon is achieved by the action of pyrrolysyl-tRNA synthetase (PylRS) together with its …

Designer amino acids, beyond the canonical 20 that are normally used by cells, can now be
site-specifically encoded into proteins in cells and organisms. This is achieved …

The bioconjugation of proteins—that is, the creation of a covalent link between a protein and
any other molecule—has been studied for decades, partly because of the numerous …

Virtually all natural proteins are built from only 20 amino acids, and while this makes
possible all the functions they perform, the ability to encode other amino acids selected for …