Single-cell microgels: technology, challenges, and applications

T Kamperman, M Karperien, S Le Gac, J Leijten - Trends in biotechnology, 2018 - cell.com
Single-cell-laden microgels effectively act as the engineered counterpart of the smallest
living building block of life: a cell within its pericellular matrix. Recent breakthroughs have …

Point-, line-, and plane-shaped cellular constructs for 3D tissue assembly

Y Morimoto, AY Hsiao, S Takeuchi - Advanced drug delivery reviews, 2015 - Elsevier
Microsized cellular constructs such as cellular aggregates and cell-laden hydrogel blocks
are attractive cellular building blocks to reconstruct 3D macroscopic tissues with spatially …

Desmosomal proteins of DSC2 and PKP1 promote cancer cells survival and metastasis by increasing cluster formation in circulatory system

K Li, R Wu, M Zhou, H Tong, KQ Luo - Science advances, 2021 - science.org
To study how cancer cells can withstand fluid shear stress (SS), we isolated SS-resistant
breast and lung cancer cells using a microfluidic circulatory system. These SS-resistant cells …

Cell armor for protection against environmental stress: Advances, challenges and applications in micro-and nanoencapsulation of mammalian cells

O Hasturk, DL Kaplan - Acta biomaterialia, 2019 - Elsevier
Unlike unicellular organisms and plant cells surrounded with a cell wall, naked plasma
membranes of mammalian cells make them more susceptible to environmental stresses …

Controllable fabrication of inhomogeneous microcapsules for triggered release by osmotic pressure

W Zhang, L Qu, H Pei, Z Qin, J Didier, Z Wu, F Bobe… - Small, 2019 - Wiley Online Library
Inhomogeneous microcapsules that can encapsulate various cargo for controlled release
triggered by osmotic shock are designed and reported. The microcapsules are fabricated …

One-step generation and purification of cell-encapsulated hydrogel microsphere with an easily assembled microfluidic device

T Zhang, H Zhang, W Zhou, K Jiang, C Liu… - … in Bioengineering and …, 2022 - frontiersin.org
Cell-laden hydrogel microspheres with uniform size show great potential for tissue repair
and drug screening applications. Droplet microfluidic systems have been widely used for the …

Microfluidic synthesis of highly spherical calcium alginate hydrogels based on external gelation using an emulsion reactant

Y Liu, N Tottori, T Nisisako - Sensors and Actuators B: Chemical, 2019 - Elsevier
Alginate-based hydrogels are widely used in the biomedical and chemical fields, and their
size and shape are significant to their applications like drug delivery and cell encapsulation …

Mass Production of Cell‐Laden Calcium Alginate Particles with Centrifugal Force

Y Morimoto, M Onuki, S Takeuchi - Advanced healthcare …, 2017 - Wiley Online Library
This paper describes a centrifuge‐based device for oil‐free and mass production of calcium‐
alginate (Ca‐alginate) particles. The device is composed of four components: a tank with a …

Electrospraying of microfluidic encapsulated cells for the fabrication of cell-laden electrospun hybrid tissue constructs

L Weidenbacher, A Abrishamkar, M Rottmar, AG Guex… - Acta biomaterialia, 2017 - Elsevier
The fabrication of functional 3D tissues is a major goal in tissue engineering. While
electrospinning is a promising technique to manufacture a structure mimicking the …

Controlled self-assembly of alginate microgels by rapidly binding molecule pairs

Y Hu, AS Mao, RM Desai, H Wang, DA Weitz… - Lab on a Chip, 2017 - pubs.rsc.org
Controlled self-assembly of cell-encapsulating microscale polymeric hydrogels (microgels)
could be advantageous in a variety of tissue engineering and regenerative medicine …