Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Extracellular vesicles (EVs) are heterogeneous lipid containers with a complex molecular
cargo comprising several populations with unique roles in biological processes. These …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Mechanobiology emerges at the crossroads of medicine, biology, biophysics and
engineering and describes how the responses of proteins, cells, tissues and organs to …

Solid–liquid interfaces are important in a range of chemical, physical and biological
processes,,–, but are often not fully understood owing to the lack of high-resolution …

This review highlights recent efforts on applying electron microscopy (EM) to soft (including
biological) nanomaterials. We will show how developments of both the hardware and …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Cryo–electron microscopy, or simply cryo-EM, refers mainly to three very different yet closely
related techniques: electron crystallography, single-particle cryo-EM, and electron …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …