The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence

J Kopeika, A Thornhill, Y Khalaf - Human reproduction update, 2015 - academic.oup.com
BACKGROUND Cryopreservation has been extensively used in assisted reproductive
technology, agriculture and conservation programmes for endangered species. The …

An update on oxidative damage to spermatozoa and oocytes

CS Opuwari, RR Henkel - BioMed research international, 2016 - Wiley Online Library
On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential
cellular functions including the function of germ cells (oocytes and spermatozoa) and …

Sperm cryopreservation: Current status and future developments

A Hungerford, HW Bakos… - Reproduction, Fertility and …, 2022 - CSIRO Publishing
The cryopreservation of spermatozoa is an important reproductive technology for the
preservation of fertility in man and animals. Since the serendipitous discovery of glycerol as …

Cryopreservation media differentially affect sperm motility, morphology and DNA integrity

G Raad, L Lteif, R Lahoud, J Azoury, J Azoury… - …, 2018 - Wiley Online Library
Introduction Human sperm freezing is very widely used for male fertility preservation. This
procedure consists in adding cryoprotectants to the spermatozoa followed by cooling and …

[HTML][HTML] Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica

M Meamar, N Zribi, M Cambi, L Tamburrino… - Fertility and sterility, 2012 - Elsevier
OBJECTIVE: To analyze the effect of cryopreservation on sperm DNA fragmentation (SDF) in
two cytometric sperm populations, PIbrighter and PIdimmer, and to test the effects of Opuntia …

Sex chromosome-dependent differential viability of human spermatozoa during prolonged incubation

YA You, WS Kwon, M Saidur Rahman… - Human …, 2017 - academic.oup.com
STUDY QUESTION Are there significant differences in the ability of X chromosome-bearing
(X) spermatozoa and Y chromosome-bearing (Y) spermatozoa to survive incubation under …

Protective effects of glutathione supplementation against oxidative stress during cryopreservation of human spermatozoa

M Ghorbani, A Vatannejad, I Khodadadi, I Amiri… - …, 2016 - ingentaconnect.com
BACKGROUND: Freeze damage is one of the most important factors which impair the
membrane and DNA integrity of sperm cells. OBJECTIVE: The present study aims to …

Effects of arginine and trehalose on post-thawed bovine sperm quality

C Öztürk, Ş Güngör, MB Ataman, MN Bucak… - Acta Veterinaria …, 2017 - akjournals.com
The present study was conducted to examine the protective role of arginine and trehalose
on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were …

Improving native human sperm freezing protection by using a modified vitrification method

D Zhou, XM Wang, RX Li, YZ Wang… - Asian Journal of …, 2021 - journals.lww.com
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa
in clinical practice. However, it has been shown to have a negative impact on sperm function …

Resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing

S Verza Jr, CM Feijo, SC Esteves - International braz j urol, 2009 - SciELO Brasil
Objective: To study the resistance of human spermatozoa to cryoinjury in repeated cycles of
thaw-refreezing by using the fast liquid nitrogen vapor method. Material and Methods …