Abstract Magnesium (Mg2+) is the most prevalent divalent intracellular cation. As co-factor in
many enzymatic reactions, Mg2+ is essential for protein synthesis, energy production, and …

Membrane proteins represent a large proportion of the proteome, but have characteristics
that are problematic for many methods in modern molecular biology (that have often been …

CorA, the major Mg 2+ uptake system in prokaryotes, is gated by intracellular Mg 2+(KD∼ 1–
2 mM). X-ray crystallographic studies of CorA show similar conformations under Mg 2+ …

Structural biology comprises a variety of tools to obtain atomic resolution data for the
investigation of macromolecules. Conventional structural methodologies including …

Styrene-maleic acid (SMA) copolymers are used to extract lipid-encased membrane proteins
from lipid bilayers in a detergent-free manner, yielding SMA lipid particles (SMALPs) …

Zinc is an essential microelement to sustain all forms of life. However, excess of zinc is toxic,
therefore dedicated import, export and storage proteins for tight regulation of the zinc …

CorA, a divalent-selective channel in the metal ion transport superfamily, is the major Mg2+-
influx pathway in prokaryotes. CorA structures in closed (Mg2+-bound), and open (Mg2+ …

Mitochondrial RNA splicing 2 (MRS2) forms a magnesium (Mg2+) entry protein channel in
mitochondria. Whereas MRS2 contains two transmembrane domains constituting a pore on …

The CorA family of proteins plays a housekeeping role in the homeostasis of divalent metal
ions in many bacteria and archaea as well as in mitochondria of eukaryotes, rendering it an …

CorA proteins belong to 2-TM-GxN family of membrane proteins, and play a major role in
Mg2+ transport in prokaryotes and eukaryotic mitochondria. The selection of substrate is …