Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) has become a key technique
for monitoring structural and dynamic aspects of proteins in solution. This approach relies on …

Several proteomics approaches are available that are defined by the level (protein or
peptide) at which analysis takes place. The most widely applied method still is bottom-up …

Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …

The aspartic protease pepsin is less specific than other endoproteinases. Because aspartic
proteases like pepsin are active at low pH, they are utilized in hydrogen deuterium …

Hydrogen/deuterium exchange coupled to mass spectrometry is an excellent technique to
study protein structure changes and interactions, especially in cases where traditional high …

This article reviews the analytical aspects of measuring hydrogen exchange by mass
spectrometry (HX MS). We describe the nature of analytical selectivity in hydrogen …

The serotonin transporter (SERT), a member of the neurotransmitter: sodium symporter
family, is responsible for termination of serotonergic signaling by re-uptake of serotonin (5 …

Although the use of hydrogen exchange (HX) mass spectrometry (MS) to study proteins and
protein conformation is now over 20 years old, the perception lingers that it still has “issues.” …

Hydrogen/deuterium exchange coupled to mass spectrometry (HXMS) utilizes enzymatic
digestion of proteins to localize the information about altered exchange patterns in protein …

Chromatographic carry-over can severely distort measurements of amide H/D exchange in
proteins analyzed by LC/MS. In this work, we explored the origin of carry-over in the online …