Nuclease-mediated editing of heteroplasmic mitochondrial DNA (mtDNA) seeks to
preferentially cleave and eliminate mutant mtDNA, leaving wild-type genomes to repopulate …

Breakthroughs in the development of programmable site-specific nucleases, including zinc-
finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) …

LAGLIDADG homing endonucleases (meganucleases) are sequence-specific DNA
cleavage enzymes used for genome engineering. Recently, meganucleases fused to …

LAGLIDADG homing endonucleases (meganucleases) are site-specific mobile
endonucleases that can be adapted for genome-editing applications. However, one problem …

DNA editing offers new possibilities in synthetic biology and biomedicine for modulation or
modification of cellular functions to organisms. However, inaccuracy in this process may …

Transcription activator-like effectors contain a DNA-binding domain organized in tandem
repeats. The repeats include two adjacent residues known as the repeat variable di-residue …

Homing endonucleases (HEs) can be used to induce targeted genome modification to
reduce the fitness of pathogen vectors such as the malaria-transmitting Anopheles gambiae …

In this study, we asked whether CpG methylation could influence the DNA binding affinity
and activity of meganucleases used for genome engineering applications. A combination of …

The high DNA specificity of homing endonucleases makes them a powerful protein scaffold
to engineer enzymes for genome manipulation. Understanding their molecular recognition …

LAGLIDADG homing endonucleases (also referred to as “meganucleases”) are compact
DNA cleaving enzymes that specifically recognize long target sequences (approximately 20 …