Advances in plant molecular farming

OO Obembe, JO Popoola, S Leelavathi… - Biotechnology advances, 2011 - Elsevier
Plant molecular farming (PMF) is a new branch of plant biotechnology, where plants are
engineered to produce recombinant pharmaceutical and industrial proteins in large …

Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures

J Xu, X Ge, MC Dolan - Biotechnology advances, 2011 - Elsevier
“Molecular farming” in plants with significant advantages in cost and safety is touted as a
promising platform for the production of complex pharmaceutical proteins. While whole-plant …

Plants as bioreactors: recent developments and emerging opportunities

AK Sharma, MK Sharma - Biotechnology advances, 2009 - Elsevier
In recent years, the use of plants as bioreactors has emerged as an exciting area of research
and significant advances have created new opportunities. The driving forces behind the …

Preventing unintended proteolysis in plant protein biofactories

M Benchabane, C Goulet, D Rivard… - Plant biotechnology …, 2008 - Wiley Online Library
Numerous reports have been published over the last decade assessing the potential of
plants as useful hosts for the heterologous expression of clinically useful proteins …

Transient expression systems for plant-derived biopharmaceuticals

TV Komarova, S Baschieri, M Donini… - Expert review of …, 2010 - Taylor & Francis
In the molecular farming area, transient expression approaches for pharmaceutical proteins
production, mainly recombinant monoclonal antibodies and vaccines, were developed …

Plants as bioreactors for the production of vaccine antigens

S Tiwari, PC Verma, PK Singh, R Tuli - Biotechnology advances, 2009 - Elsevier
Plants have been identified as promising expression systems for commercial production of
vaccine antigens. In phase I clinical trials several plant-derived vaccine antigens have been …

Hydrophobin Fusions for High-Level Transient Protein Expression and Purification in Nicotiana benthamiana

JJ Joensuu, AJ Conley, M Lienemann… - Plant …, 2010 - academic.oup.com
Insufficient accumulation levels of recombinant proteins in plants and the lack of efficient
purification methods for recovering these valuable proteins have hindered the development …

[HTML][HTML] On the way to commercializing plant cell culture platform for biopharmaceuticals: present status and prospect

J Xu, N Zhang - Pharmaceutical bioprocessing, 2014 - ncbi.nlm.nih.gov
Plant cell culture is emerging as an alternative bioproduction system for recombinant
pharmaceuticals. Growing plant cells in vitro under controlled environmental conditions …

Protein body‐inducing fusions for high‐level production and purification of recombinant proteins in plants

AJ Conley, JJ Joensuu, A Richman… - Plant Biotechnology …, 2011 - Wiley Online Library
For the past two decades, therapeutic and industrially important proteins have been
expressed in plants with varying levels of success. The two major challenges hindering the …

N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential

KT Hamorsky, JC Kouokam, JM Jurkiewicz… - Scientific Reports, 2015 - nature.com
Plant-based transient overexpression systems enable rapid and scalable production of
subunit vaccines. Previously, we have shown that cholera toxin B subunit (CTB), an oral …