The origins of low-temperature tissue storage research date back to the late 1800s. Over half
a century later, osmotic stress was revealed to be a main contributor to cell death during …

Cryopreservation has facilitated advancement of biological research by allowing the storage
of cells over prolonged periods of time. While cryopreservation at extremely low …

Stem cells have been used in a clinical setting for many years. Haematopoietic stem cells
have been used for the treatment of both haematological and non-haematological disease; …

Pluripotent stem cells have the capacity to self renew and to differentiate to cells of the three
somatic germ layers that comprise an organism. Embryonic stem cells are the most studied …

Human embryonic stem cell (hESC) lines, after directed differentiation, hold the greatest
potential for cell transplantation treatment in many severe diseases. Good manufacturing …

Stem cell therapy offers tremendous promise in the treatment of many incurable diseases. A
variety of stem cell types are being studied but human embryonic stem cells (hESCs) appear …

BACKGROUND Efficient slow freezing protocols within serum-free and feeder-free culture
systems are crucial for the clinical application of human embryonic stem (hES) cells …

Cryopreservation is the application of low temperatures to preserve the structural and
functional integrity of cells and tissues. Conventional cooling protocols allow ice to form and …

As a result of their pluripotency and potential for unlimited self‐renewal, human embryonic
stem cells (hESCs) hold tremendous promise in regenerative medicine. An essential …

Cryopreservation is the most prevalent method of long‐term cell preservation. Effective cell
cryopreservation depends on freezing, adequate storage, and correct thawing techniques …