Introduction Cellulases are of great interest for application in biomass degradation, yet the
molecular details of the mode of action of glycoside hydrolases during degradation of …

A 100‐kDa protein with endoglucanase activity was purified from Triton X‐100 extract of
cells of the thermoacidophilic Gram‐positive bacterium Alicyclobacillus acidocaldarius. The …

Caulobacter crescentus (C. crescentus) has a single celA gene encoding the bacterial
cellulase (EC 3.2. 1.4) of the GH9 family that is induced at transcriptional levels (q-PCR) by …

The production of biofuels using biomass is an alternative route to support the growing
global demand for energy and to also reduce the environmental problems caused by the …

The NAC domain (residues 1–168) of ANAC, encoded by the abscisic acid-responsive NAC
gene from Arabidopsis thaliana, was recombinantly produced in Escherichia coli and …

Alicyclobacillus acidocaldarius endoglucanase Cel9A (AaCel9A) is an inverting glycoside
hydrolase with β-1, 4-glucanase activity on soluble polymeric substrates. Here, we report …

Cel9A from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius belongs to the
subfamily E1 of family 9 glycoside hydrolases, many members of which have an N-terminal …

Glycoside hydrolase family 9 (GH9) endoglucanases are important enzymes for cellulose
degradation. However, their activity on cellulose is diverse. Here, we cloned and expressed …

Protein crystallization screens frequently yield salt crystals as well as protein crystals. A
simple method for determining whether a crystal is composed of salt or macromolecules is …

Confocal microscopy, a technique that has been extensively applied in cellular biological
studies, may also be applied to the visualization and three-dimensional imaging of protein …