The emerging landscape of single-molecule protein sequencing technologies

JA Alfaro, P Bohländer, M Dai, M Filius, CJ Howard… - Nature …, 2021 - nature.com
Single-cell profiling methods have had a profound impact on the understanding of cellular
heterogeneity. While genomes and transcriptomes can be explored at the single-cell level …

Engineering biological nanopore approaches toward protein sequencing

X Wei, T Penkauskas, JE Reiner, C Kennard… - ACS …, 2023 - ACS Publications
Biotechnological innovations have vastly improved the capacity to perform large-scale
protein studies, while the methods we have for identifying and quantifying individual proteins …

Solid-state nanopore systems: from materials to applications

Y He, M Tsutsui, Y Zhou, XS Miao - NPG Asia Materials, 2021 - nature.com
Ion transport and hydrodynamic flow through nanometer-sized channels (nanopores) have
been increasingly studied owing to not only the fundamental interest in the abundance of …

Nanopores in graphene and other 2D materials: a decade's journey toward sequencing

H Qiu, W Zhou, W Guo - ACS nano, 2021 - ACS Publications
Nanopore techniques offer a low-cost, label-free, and high-throughput platform that could be
used in single-molecule biosensing and in particular DNA sequencing. Since 2010 …

Hydrophobically gated memristive nanopores for neuromorphic applications

G Paulo, K Sun, G Di Muccio, A Gubbiotti… - Nature …, 2023 - nature.com
Signal transmission in the brain relies on voltage-gated ion channels, which exhibit the
electrical behaviour of memristors, resistors with memory. State-of-the-art technologies …

Portable nanopore-sequencing technology: Trends in development and applications

P Chen, Z Sun, J Wang, X Liu, Y Bai, J Chen… - Frontiers in …, 2023 - frontiersin.org
Sequencing technology is the most commonly used technology in molecular biology
research and an essential pillar for the development and applications of molecular biology …

Protein Sizing with 15 nm Conical Biological Nanopore YaxAB

S Straathof, G Di Muccio, M Yelleswarapu… - ACS …, 2023 - ACS Publications
Nanopores are promising single-molecule tools for the electrical identification and
sequencing of biomolecules. However, the characterization of proteins, especially in real …

Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) nanopore by a helicase motor for peptide sequencing …

Z Chen, Z Wang, Y Xu, X Zhang, B Tian, J Bai - Chemical science, 2021 - pubs.rsc.org
The lack of an efficient, low-cost sequencing method has long been a significant bottleneck
in protein research and applications. In recent years, the nanopore platform has emerged as …

Analytical techniques for multiplex analysis of protein biomarkers

A Van Gool, F Corrales, M Čolović, D Krstić… - Expert review of …, 2020 - Taylor & Francis
Introduction The importance of biomarkers for pharmaceutical drug development and clinical
diagnostics is more significant than ever in the current shift toward personalized medicine …

Electroosmosis in nanopores: computational methods and technological applications

A Gubbiotti, M Baldelli, G Di Muccio… - … in Physics: X, 2022 - Taylor & Francis
Electroosmosis is a fascinating effect where liquid motion is induced by an applied electric
field. Counter ions accumulate in the vicinity of charged surfaces, triggering a coupling …