Mitochondrial function and redox state in mammalian embryos

R Dumollard, J Carroll, MR Duchen, K Campbell… - Seminars in cell & …, 2009 - Elsevier
Mitochondria play a central and multifaceted role in the mammalian egg and early embryo,
contributing to many different aspects of early development. While the contribution of …

Tissue injury and repair in the female human reproductive tract

LA Salamonsen - Reproduction, 2003 - rep.bioscientifica.com
The major endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) is a member of the
endocannabinoid system (ECS) that participates in cell proliferation and apoptosis …

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

DK Gardner, RL Kelley - Journal of Developmental Origins of Health …, 2017 - cambridge.org
The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its
morphology, developmental kinetics, physiology and metabolism, can be affected by …

[HTML][HTML] The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study

MH Kang, J Das, S Gurunathan, HW Park, H Song… - Theranostics, 2017 - ncbi.nlm.nih.gov
Rationale: Dimethyl sulfoxide (DMSO) is commonly used as a solvent for water-insoluble
substances, a vehicle for drug therapy, and a cryoprotectant for cultured cells. DMSO …

Cell volume regulation in oocytes and early embryos: connecting physiology to successful culture media

JM Baltz, AP Tartia - Human reproduction update, 2010 - academic.oup.com
BACKGROUND Preimplantation embryos are particularly susceptible to in vitro
developmental blocks. These could be alleviated by lowering culture medium osmolarity …

Production of Pigs From Porcine Embryos Generated in vitro

PR Chen, K Uh, BK Redel, ED Reese… - Frontiers in Animal …, 2022 - frontiersin.org
Generating porcine embryos in vitro is a critical process for creating genetically modified
pigs as agricultural and biomedical models; however, these embryo technologies have been …

Microdrop preparation factors influence culture-media osmolality, which can impair mouse embryo preimplantation development

JE Swain, L Cabrera, X Xu, GD Smith - Reproductive biomedicine online, 2012 - Elsevier
Because media osmolality can impact embryo development, the effect of conditions during
microdrop preparation on osmolality was examined. Various sizes of microdrops were …

Optimal human embryo culture

JE Swain - Seminars in reproductive medicine, 2015 - thieme-connect.com
A large contributor to success during in vitro fertilization (IVF) lies in the processes occurring
within the IVF laboratory. These processes make up the “culture system.” This system entails …

Variable reprogramming of the pluripotent stem cell marker Oct4 in mouse clones: distinct developmental potentials in different culture environments

M Boiani, L Gentile, VV Gambles, F Cavaleri… - Stem Cells, 2005 - academic.oup.com
A prevailing view of cloning by somatic‐cell nuclear transfer is that reprogramming of gene
expression occurs during the first few hours after injection of the nucleus into an oocyte, that …

A propensity score-based, comparative study assessing humid and dry time-lapse incubation, with single-step medium, on embryo development and clinical outcomes

MÁ Valera, C Albert, J Marcos, Z Larreategui… - Human …, 2022 - academic.oup.com
STUDY QUESTION Does culture in a high relative humidity atmosphere improve clinical
outcomes when using a time-lapse integrated incubator and single-step culture medium …