Critical review of 16S rRNA gene sequencing workflow in microbiome studies: From primer selection to advanced data analysis

A Regueira‐Iglesias, C Balsa‐Castro… - Molecular Oral …, 2023 - Wiley Online Library
The multi‐batch reanalysis approach of jointly reevaluating gene/genome sequences from
different works has gained particular relevance in the literature in recent years. The large …

Therapeutic Modulation of the Microbiome in Oncology: Current Trends and Future Directions

I Saraswat, A Goel - Current Pharmaceutical Biotechnology, 2024 - benthamdirect.com
Cancer is a predominant cause of mortality worldwide, necessitating the development of
innovative therapeutic techniques. The human microbiome, particularly the gut microbiota …

[HTML][HTML] Characterization of the archaeal community in foods: The neglected part of the food microbiota

H Mohammadpour, M Cardin, L Carraro… - International Journal of …, 2023 - Elsevier
Despite the large number of studies conducted on archaea associated with extreme
environments, the archaeal community composition in food products is still poorly known …

Long journey of 16S rRNA‐amplicon sequencing toward cell‐based functional bacterial microbiota characterization

J Jin, X Liu, K Shiroguchi - iMetaOmics, 2024 - Wiley Online Library
Bacteria often exist and function as a community, known as the bacterial microbiota, which
consists of vast numbers of bacteria belonging to many bacterial species (taxa) …

Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and database

T Nagai, T Shiba, K Komatsu, T Watanabe… - Microbiology …, 2024 - journals.asm.org
ABSTRACT 16S rRNA gene amplicon sequencing analysis is used to investigate bacterial
communities; however, the estimated bacterial composition can differ from the original due …

In-Silico Detection of Oral Prokaryotic Species With Highly Similar 16S rRNA Sequence Segments Using Different Primer Pairs

A Regueira-Iglesias, L Vázquez-González… - Frontiers in Cellular …, 2022 - frontiersin.org
Although clustering by operational taxonomic units (OTUs) is widely used in the oral
microbial literature, no research has specifically evaluated the extent of the limitations of this …

OTUs clustering should be avoided for defining oral microbiome

A Regueira-Iglesias, L Vázquez-González… - bioRxiv, 2021 - biorxiv.org
This in silico investigation aimed to: 1) evaluate a set of primer pairs with high coverage,
including those most commonly used in the literature, to find the different oral species with …

Microbiological diagnostics: From traditional to molecular genetic methods: A literature review

M Ivashko, S Burmei, L Yusko, T Chaikovska, N Boyko - 2023 - dspace.uzhnu.edu.ua
The development of new and optimisation of known cultural and molecular genetic methods
for accurate species identification of microorganisms is an urgent and practically necessary …