Single-cell analysis tools for drug discovery and development
The genetic, functional or compositional heterogeneity of healthy and diseased tissues
presents major challenges in drug discovery and development. Such heterogeneity hinders …
presents major challenges in drug discovery and development. Such heterogeneity hinders …
Scaling by shrinking: empowering single-cell'omics' with microfluidic devices
Recent advances in cellular profiling have demonstrated substantial heterogeneity in the
behaviour of cells once deemed'identical', challenging fundamental notions of …
behaviour of cells once deemed'identical', challenging fundamental notions of …
Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-
input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for …
input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for …
Single-cell technologies for monitoring immune systems
PK Chattopadhyay, TM Gierahn, M Roederer… - Nature …, 2014 - nature.com
The complex heterogeneity of cells, and their interconnectedness with each other, are major
challenges to identifying clinically relevant measurements that reflect the state and capability …
challenges to identifying clinically relevant measurements that reflect the state and capability …
Functional TCR T cell screening using single-cell droplet microfluidics
Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer
immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone …
immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone …
Classification of human natural killer cells based on migration behavior and cytotoxic response
B Vanherberghen, PE Olofsson… - Blood, The Journal …, 2013 - ashpublications.org
Despite intense scrutiny of the molecular interactions between natural killer (NK) and target
cells, few studies have been devoted to dissection of the basic functional heterogeneity in …
cells, few studies have been devoted to dissection of the basic functional heterogeneity in …
Single-cell multiplexed cytokine profiling of CD19 CAR-T cells reveals a diverse landscape of polyfunctional antigen-specific response
Q Xue, E Bettini, P Paczkowski, C Ng, A Kaiser… - … for immunotherapy of …, 2017 - Springer
Background It remains challenging to characterize the functional attributes of chimeric
antigen receptor (CAR)-engineered T cell product targeting CD19 related to potency and …
antigen receptor (CAR)-engineered T cell product targeting CD19 related to potency and …
Evaluation of single-cell cytokine secretion and cell-cell interactions with a hierarchical loading microwell chip
Comprehensive evaluation of single T cell functions such as cytokine secretion and cytolysis
of target cells is greatly needed in adoptive cell therapy (ACT) but has never been fully …
of target cells is greatly needed in adoptive cell therapy (ACT) but has never been fully …
Advancing single-cell proteomics and metabolomics with microfluidic technologies
Recent advances in single-cell analysis have unraveled substantial heterogeneity among
seemingly identical cells at genomic and transcriptomic levels. These discoveries have …
seemingly identical cells at genomic and transcriptomic levels. These discoveries have …
Antibody Fc engineering improves frequency and promotes kinetic boosting of serial killing mediated by NK cells
G Romain, V Senyukov… - Blood, The Journal …, 2014 - ashpublications.org
The efficacy of most therapeutic monoclonal antibodies (mAbs) targeting tumor antigens
results primarily from their ability to elicit potent cytotoxicity through effector-mediated …
results primarily from their ability to elicit potent cytotoxicity through effector-mediated …