Viruses that infect bacteria (bacteriophages; also known as phages) were discovered 100
years ago. Since then, phage research has transformed fundamental and translational …

Gene essentiality is a founding concept of genetics with important implications in both
fundamental and applied research. Multiple screens have been performed over the years in …

We have developed a new vector strategy for the insertion of foreign genes into the
genomes of gram negative bacteria not closely related to Escherichia coli. The system …

Our knowledge of gene function has increasingly lagged behind gene discovery, hindering
our understanding of the genetic basis of microbial phenotypes. Recently, however …

An auxotrophic mutant which requires a metabolite not available in vertebrate tissues should
be unable to grow in such tissue and thus be non-virulent. Most mutations to auxotrophy do …

Recombinant E. coli that produce light were found in a clone library of hybrid plasmids
containing DNA from the marine bacterium Vibrio fischeri. All luminescent clones had a 16 …

The development of plant tissue (including organ and cell) cultures for the production of
secondary metabolites has been underway for more than three decades. Plant cell cultures …

Salmonella typhimurium is a facultative intracellular pathogen capable of surviving within
phagocytic cells of the reticuloendothelial system. To identify the genes important for …

METHODS Pl~ mid constructions. Plasmid pOX38 was isolated as a conjugative replicon
composed of the largest Hin-dlII digestion fragment of F. F was completely digested with …

A fundamental goal of genetics and functional genomics is to identify and mutate every gene
in model organisms such as Drosophila melanogaster. The Berkeley Drosophila Genome …