Next-generation sequencing: insights to advance clinical investigations of the microbiome

CR Wensel, JL Pluznick, SL Salzberg… - The Journal of clinical …, 2022 - Am Soc Clin Investig
Next-generation sequencing (NGS) technology has advanced our understanding of the
human microbiome by allowing for the discovery and characterization of unculturable …

Mass spectrometry-based metabolomics in microbiome investigations

A Bauermeister, H Mannochio-Russo… - Nature Reviews …, 2022 - nature.com
Microbiotas are a malleable part of ecosystems, including the human ecosystem.
Microorganisms affect not only the chemistry of their specific niche, such as the human gut …

MicrobiomeAnalyst 2.0: comprehensive statistical, functional and integrative analysis of microbiome data

Y Lu, G Zhou, J Ewald, Z Pang, T Shiri… - Nucleic Acids …, 2023 - academic.oup.com
Microbiome studies have become routine in biomedical, agricultural and environmental
sciences with diverse aims, including diversity profiling, functional characterization, and …

Multi-level analysis of the gut–brain axis shows autism spectrum disorder-associated molecular and microbial profiles

JT Morton, DM Jin, RH Mills, Y Shao, G Rahman… - Nature …, 2023 - nature.com
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by
heterogeneous cognitive, behavioral and communication impairments. Disruption of the gut …

Greengenes2 unifies microbial data in a single reference tree

D McDonald, Y Jiang, M Balaban, K Cantrell… - Nature …, 2024 - nature.com
Studies using 16S rRNA and shotgun metagenomics typically yield different results, usually
attributed to PCR amplification biases. We introduce Greengenes2, a reference tree that …

DS-1000: A natural and reliable benchmark for data science code generation

Y Lai, C Li, Y Wang, T Zhang, R Zhong… - International …, 2023 - proceedings.mlr.press
We introduce DS-1000, a code generation benchmark with a thousand data science
problems spanning seven Python libraries, such as Numpy and Pandas. Compared to prior …

A gut-derived metabolite alters brain activity and anxiety behaviour in mice

BD Needham, M Funabashi, MD Adame, Z Wang… - Nature, 2022 - nature.com
Integration of sensory and molecular inputs from the environment shapes animal behaviour.
A major site of exposure to environmental molecules is the gastrointestinal tract, in which …

The microbiome-derived metabolite TMAO drives immune activation and boosts responses to immune checkpoint blockade in pancreatic cancer

G Mirji, A Worth, SA Bhat, M El Sayed, T Kannan… - Science …, 2022 - science.org
The composition of the gut microbiome can control innate and adaptive immunity and has
emerged as a key regulator of tumor growth, especially in the context of immune checkpoint …

Gut microbiota from green tea polyphenol-dosed mice improves intestinal epithelial homeostasis and ameliorates experimental colitis

Z Wu, S Huang, T Li, N Li, D Han, B Zhang, ZZ Xu… - Microbiome, 2021 - Springer
Background Alteration of the gut microbiota may contribute to the development of
inflammatory bowel disease (IBD). Epigallocatechin-3-gallate (EGCG), a major bioactive …

Microbiome differential abundance methods produce different results across 38 datasets

JT Nearing, GM Douglas, MG Hayes… - Nature …, 2022 - nature.com
Identifying differentially abundant microbes is a common goal of microbiome studies.
Multiple methods are used interchangeably for this purpose in the literature. Yet, there are …