Protoplasts: from isolation to CRISPR/Cas genome editing application

JJ Yue, JL Yuan, FH Wu, YH Yuan… - Frontiers in Genome …, 2021 - frontiersin.org
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR
associated protein (Cas) system, protoplasts are not only useful for rapidly validating the …

Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants

C Chen, Y Ge, L Lu - Frontiers in Plant Science, 2023 - frontiersin.org
Single-cell and spatial transcriptomics have diverted researchers' attention from the
multicellular level to the single-cell level and spatial information. Single-cell transcriptomes …

Multiplex CRISPR editing of wood for sustainable fiber production

DB Sulis, X Jiang, C Yang, BM Marques, ML Matthews… - Science, 2023 - science.org
The domestication of forest trees for a more sustainable fiber bioeconomy has long been
hindered by the complexity and plasticity of lignin, a biopolymer in wood that is recalcitrant to …

Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

JP Wang, ML Matthews, CM Williams, R Shi… - Nature …, 2018 - nature.com
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the
strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three …

CRISPR–Cas9-mediated genome editing in apple and grapevine

Y Osakabe, Z Liang, C Ren, C Nishitani, K Osakabe… - Nature …, 2018 - nature.com
The CRISPR–Cas9 genome-editing tool and the availability of whole-genome sequences
from plant species have revolutionized our ability to introduce targeted mutations into …

Combining single-cell RNA sequencing with spatial transcriptome analysis reveals dynamic molecular maps of cambium differentiation in the primary and secondary …

R Li, Z Wang, JW Wang, L Li - Plant Communications, 2023 - cell.com
Primary and secondary growth of the tree stem are responsible for corresponding increases
in trunk height and diameter. However, our molecular understanding of the biological …

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa

X Dai, R Zhai, J Lin, Z Wang, D Meng, M Li, Y Mao… - Nature plants, 2023 - nature.com
Stem vascular cambium cells in forest trees produce wood for materials and energy. WOX4
affects the proliferation of such cells in Populus. Here we show that Ptr WOX4a is the most …

Root‐specific NF‐Y family transcription factor, PdNF‐YB21, positively regulates root growth and drought resistance by abscisic acid‐mediated indoylacetic acid …

Y Zhou, Y Zhang, X Wang, X Han, Y An, S Lin… - New …, 2020 - Wiley Online Library
Root growth control plays an important role in plant adaptation to drought stress, but the
underlying molecular mechanisms of this control remain largely elusive. Here, a root …

The AREB1 Transcription Factor Influences Histone Acetylation to Regulate Drought Responses and Tolerance in Populus trichocarpa

S Li, YCJ Lin, P Wang, B Zhang, M Li, S Chen… - The Plant …, 2019 - academic.oup.com
Plants develop tolerance to drought by activating genes with altered levels of epigenetic
modifications. Specific transcription factors are involved in this activation, but the molecular …

PuC3H35 confers drought tolerance by enhancing lignin and proanthocyanidin biosynthesis in the roots of Populus ussuriensis

D Li, J Yang, S Pak, M Zeng, J Sun, S Yu… - New …, 2022 - Wiley Online Library
Since the roots are the very organ where plants first sense and respond drought stress, it is
of great importance to better understand root responses to drought. Yet the underlying …