Metabolic flux analysis (MFA) is one of the pillars of metabolic engineering. Over the past
three decades, it has been widely used to quantify intracellular metabolic fluxes in both …

The paper gives a review on the recent development of bioprocess engineering. It includes
monitoring of product formation processes by flow injection analysis, various types of …

Oleaginous microbes represent an attractive means of converting a diverse range of
feedstocks into oils that can be transesterified to biodiesel. However, the mechanism of lipid …

Pentose phosphate pathway and isocitrate dehydrogenase are generally considered to be
the major sources of the anabolic reductant NADPH. As one of very few microbes …

Metabolic fluxes, estimated from stable isotope studies, provide a key to quantifying
physiology in fields ranging from metabolic engineering to the analysis of human metabolic …

A general methodology is presented for the modeling, simulation, design, evaluation, and
statistical analysis of 13C-labeling experiments for metabolic flux analysis. The universal …

Recent advances in metabolic flux analysis including genome-scale constraints-based flux
analysis and its applications in metabolic engineering are reviewed. Various computational …

Detailed knowledge on carbon flux distributions is crucial for the understanding and targeted
optimization of cellular systems. Analytical methods to identify the topology of metabolic …

Metabolic flux analysis via 13C labeling (13C MFA) quantitatively tracks metabolic pathway
activity and determines overall enzymatic function in cells. Three core techniques are …

Volatile fatty acids (VFAs) are an inexpensive and renewable carbon source that can be
generated from gas fermentation and anaerobic digestion of fermentable wastes. The …