Interactions between newly integrate DNA and the host genome limit the reliability and
safety of transgene integration for therapeutic cell engineering and other applications …

Genomic safe harbors (GSHs) are sites in the genome able to accommodate the integration
of new genetic material in a manner that ensures that the newly inserted genetic elements:(i) …

Studying the role of essential proteins is dependent upon a method for rapid inactivation, in
order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) …

Effective and safe delivery of the CRISPR/Cas9 gene-editing elements remains a challenge.
Here we report the development of PEGylated nanoparticles (named P-HNPs) based on the …

Human pluripotent stem cells (hPSCs) offer a unique platform for elucidating the genes and
molecular pathways that underlie complex traits and diseases. To realize this promise …

Neutrophils, the most abundant white blood cells in circulation, are closely related to cancer
development and progression. Healthy primary neutrophils present potent cytotoxicity …

Precise studies of plant, animal and human genomes enable remarkable opportunities of
obtained data application in biotechnology and medicine. However, knowing nucleotide …

Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent
stem cells (hiPSCs) requires efficient methods for genetic modification. However, techniques …

The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid
donor DNA | Journal of Experimental Medicine | Rockefeller University Press Skip to Main …