Target-identification and mechanism-of-action studies have important roles in small-
molecule probe and drug discovery. Biological and technological advances have resulted in …

The zebrafish model is the only available high-throughput vertebrate assessment system,
and it is uniquely suited for studies of in vivo cell biology. A sequenced and annotated …

The widespread availability of programmable site-specific nucleases now enables targeted
gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate …

Despite three decades of successful, predominantly phenotype-driven discovery of the
genetic causes of monogenic disorders, up to half of children with severe developmental …

Animal models are essential tools for addressing fundamental scientific questions about
skeletal diseases and for the development of new therapeutic approaches. Traditionally …

Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering
RNAs (siRNAs) are commonly used platforms to study gene function by sequence-specific …

Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used
in zebrafish (Danio rerio). This review discusses common practices for the design …

The development of facile forward and reverse genetic approaches has propelled the
deconvolution of gene function in biology. While the origins of these techniques reside in the …

The global obesity epidemic demands an improved understanding of the developmental
and environmental factors regulating fat storage. Adipocytes serve as major sites of fat …

Morpholino oligonucleotides (MOs) are an effective, gene-specific antisense knockdown
technology used in many model systems. Here we describe the application of MOs in …