Perturbations of native membrane protein structure in alkyl phosphocholine detergents: a critical assessment of NMR and biophysical studies
Membrane proteins perform a host of vital cellular functions. Deciphering the molecular
mechanisms whereby they fulfill these functions requires detailed biophysical and structural …
mechanisms whereby they fulfill these functions requires detailed biophysical and structural …
Methods and applications of site-directed spin labeling EPR spectroscopy
Site-directed spin labeling (SDSL) electron paramagnetic resonance (EPR) spectroscopy
has emerged as a well-established method that can provide specific information on the …
has emerged as a well-established method that can provide specific information on the …
Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy
OC Andronesi, S Becker, K Seidel… - Journal of the …, 2005 - ACS Publications
It is shown that molecular structure and dynamics of a uniformly labeled membrane protein
can be studied under magic-angle-spinning conditions. For this purpose, dipolar recoupling …
can be studied under magic-angle-spinning conditions. For this purpose, dipolar recoupling …
Internal dynamics of a supramolecular nanofibre
A large variety of functional self-assembled supramolecular nanostructures have been
reported over recent decades. The experimental approach to these systems initially focused …
reported over recent decades. The experimental approach to these systems initially focused …
Cell death versus cell survival instructed by supramolecular cohesion of nanostructures
Many naturally occurring peptides containing cationic and hydrophobic domains have
evolved to interact with mammalian cell membranes and have been incorporated into …
evolved to interact with mammalian cell membranes and have been incorporated into …
Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach
Phospholamban (PLN) is an essential regulator of cardiac muscle contractility. The
homopentameric assembly of PLN is the reservoir for active monomers that, upon …
homopentameric assembly of PLN is the reservoir for active monomers that, upon …
Functions of intrinsic disorder in transmembrane proteins
M Kjaergaard, BB Kragelund - Cellular and Molecular Life Sciences, 2017 - Springer
Intrinsic disorder is common in integral membrane proteins, particularly in the intracellular
domains. Despite this observation, these domains are not always recognized as being …
domains. Despite this observation, these domains are not always recognized as being …
Structural and Dynamic Basis of Phospholamban and Sarcolipin Inhibition of Ca2+-ATPase
Phospholamban (PLN) and sarcolipin (SLN) are two single-pass membrane proteins that
regulate Ca2+-ATPase (SERCA), an ATP-driven pump that translocates calcium ions into …
regulate Ca2+-ATPase (SERCA), an ATP-driven pump that translocates calcium ions into …
Phosphorylation-dependent conformational switch in spin-labeled phospholamban bound to SERCA
CB Karim, Z Zhang, EC Howard, KD Torgersen… - Journal of molecular …, 2006 - Elsevier
We have used chemical synthesis, functional reconstitution, and electron paramagnetic
resonance (EPR) to probe the functional dynamics of phospholamban (PLB), which …
resonance (EPR) to probe the functional dynamics of phospholamban (PLB), which …
Targeting protein-protein interactions for therapeutic discovery via FRET-based high-throughput screening in living cells
DR Stroik, SL Yuen, KA Janicek, TM Schaaf, J Li… - Scientific reports, 2018 - nature.com
We have developed a structure-based high-throughput screening (HTS) method, using time-
resolved fluorescence resonance energy transfer (TR-FRET) that is sensitive to protein …
resolved fluorescence resonance energy transfer (TR-FRET) that is sensitive to protein …