Versatile trityl spin labels for nanometer distance measurements on biomolecules in vitro and within cells

JJ Jassoy, A Berndhäuser, F Duthie… - Angewandte …, 2017 - Wiley Online Library
Abstract Structure determination of biomacromolecules under in‐cell conditions is a relevant
yet challenging task. Electron paramagnetic resonance (EPR) distance measurements in …

Physiological-temperature distance measurement in nucleic acid using triarylmethyl-based spin labels and pulsed dipolar EPR spectroscopy

GY Shevelev, OA Krumkacheva… - Journal of the …, 2014 - ACS Publications
Resolving the nanometer-scale structure of biomolecules in natural conditions still remains a
challenging task. We report the first distance measurement in nucleic acid at physiological …

Trityl radicals: synthesis, properties, and applications

VM Tormyshev, EG Bagryanskaya - Russian Chemical Bulletin, 2021 - Springer
Trityl radicals: synthesis, properties, and applications* Page 1 Russian Chemical Bulletin,
International Edition, Vol. 70, No. 12, pp. 2278—2297, December, 2021 2278 Published in …

Three-spin solid effect and the spin diffusion barrier in amorphous solids

KO Tan, M Mardini, C Yang, JH Ardenkjær-Larsen… - Science …, 2019 - science.org
Dynamic nuclear polarization (DNP) has evolved as the method of choice to enhance NMR
signal intensities and to address a variety of otherwise inaccessible chemical, biological and …

Solid-effect dynamic nuclear polarization in viscous liquids at 9.4 T using narrow-line polarizing agents

AA Kuzhelev, V Denysenkov, IM Ahmad… - Journal of the …, 2023 - ACS Publications
Dynamic nuclear polarization (DNP) is a hyperpolarization method that is widely used for
increasing the sensitivity of nuclear magnetic resonance (NMR) experiments. DNP is …

Room-temperature electron spin relaxation of triarylmethyl radicals at the X-and Q-bands

AA Kuzhelev, DV Trukhin… - The Journal of …, 2015 - ACS Publications
Triarylmethyl radicals (trityls, TAMs) represent a relatively new class of spin labels. The long
relaxation of trityls at room temperature in liquid solutions makes them a promising …

Compaction of RNA Duplexes in the Cell

A Collauto, S von Bülow, DB Gophane… - Angewandte …, 2020 - Wiley Online Library
The structure and flexibility of RNA depends sensitively on the microenvironment. Using
pulsed electron‐electron double‐resonance (PELDOR)/double electron‐electron resonance …

Spin labels for 19 F ENDOR distance determination: resolution, sensitivity and distance predictability

A Bogdanov, L Gao, A Dalaloyan, W Zhu… - Physical Chemistry …, 2024 - pubs.rsc.org
19F electron-nuclear double resonance (ENDOR) has emerged as an attractive method for
determining distance distributions in biomolecules in the range of 0.7–2 nm, which is not …

A 13C‐Labeled Triarylmethyl Radical as an EPR Spin Probe Highly Sensitive to Molecular Tumbling

M Poncelet, B Driesschaert - Angewandte Chemie, 2020 - Wiley Online Library
A stable triarylmethyl spin probe whose electron paramagnetic resonance (EPR) spectrum is
highly sensitive to molecular tumbling is reported. The strong anisotropy of the hyperfine …

EPR-based distance measurements at ambient temperature

O Krumkacheva, E Bagryanskaya - Journal of Magnetic Resonance, 2017 - Elsevier
Pulsed dipolar (PD) EPR spectroscopy is a powerful technique allowing for distance
measurements between spin labels in the range of 2.5–10.0 nm. It was proposed more than …