Recent advances in microscale electroporation

SE Choi, H Khoo, SC Hur - Chemical reviews, 2022 - ACS Publications
Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …

Moyamoya disease emerging as an immune-related angiopathy

C Asselman, D Hemelsoet, D Eggermont… - Trends in Molecular …, 2022 - cell.com
Moyamoya disease (MMD) is a rare cerebrovascular disorder with unknown etiology. MMD
is characterized by progressive narrowing of arteries of the brain and the formation of a …

Isotachophoresis: theory and microfluidic applications

A Ramachandran, JG Santiago - Chemical Reviews, 2022 - ACS Publications
Isotachophoresis (ITP) is a versatile electrophoretic technique that can be used for sample
preconcentration, separation, purification, and mixing, and to control and accelerate …

Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy

ML Richter, IK Deligiannis, K Yin, A Danese… - Nature …, 2021 - nature.com
Single-cell RNA-seq reveals the role of pathogenic cell populations in development and
progression of chronic diseases. In order to expand our knowledge on cellular …

Single cell transcriptome profiling of the human alcohol-dependent brain

E Brenner, GR Tiwari, M Kapoor, Y Liu… - Human Molecular …, 2020 - academic.oup.com
Alcoholism remains a prevalent health concern throughout the world. Previous studies have
identified transcriptomic patterns in the brain associated with alcohol dependence in both …

Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels

WTC Uniken Venema, AD Ramírez-Sánchez… - Scientific Reports, 2022 - nature.com
Single-cell RNA sequencing (scRNA-seq) has revolutionized the study of the cellular
landscape of organs. Most single-cell protocols require fresh material, which limits sample …

Tissue-specific transcriptome profiling of the Arabidopsis inflorescence stem reveals local cellular signatures

D Shi, V Jouannet, J Agustí, V Kaul, V Levitsky… - The Plant …, 2021 - academic.oup.com
Genome-wide gene expression maps with a high spatial resolution have substantially
accelerated plant molecular science. However, the number of characterized tissues and …

Frontiers in single cell analysis: multimodal technologies and their clinical perspectives

J Källberg, W Xiao, D Van Assche, JC Baret, V Taly - Lab on a Chip, 2022 - pubs.rsc.org
Single cell multimodal analysis is at the frontier of single cell research: it defines the roles
and functions of distinct cell types through simultaneous analysis to provide unprecedented …

Oligodendrocyte lineage cells: Advances in development, disease, and heterogeneity

JQ Wang, MY Gao, R Gao, KH Zhao… - Journal of …, 2023 - Wiley Online Library
Oligodendrocyte progenitor cells (OPCs) originate in the ventricular zone (VZ) of the brain
and spinal cord, and their primary function is to differentiate into oligodendrocytes (OLs) …

Single-nucleus and spatial transcriptomics of archival pancreatic cancer reveals multi-compartment reprogramming after neoadjuvant treatment

WL Hwang, KA Jagadeesh, JA Guo, HI Hoffman… - BioRxiv, 2020 - biorxiv.org
Pancreatic ductal adenocarcinoma (PDAC) remains a treatment-refractory disease.
Characterizing PDAC by mRNA profiling remains particularly challenging. Previously …