Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

L Li, S Wu, Y Si, H Li, X Yin… - Comprehensive Reviews in …, 2022 - Wiley Online Library
Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …

Advances in chicken IgY-based immunoassays for the detection of chemical and biological hazards in food samples

L Dou, Y Zhang, Y Bai, Y Li, M Liu, S Shao… - Journal of Agricultural …, 2022 - ACS Publications
As antibodies are the main biological binder for hazards in food samples, their performance
directly determines the sensitivity, specificity, and reproducibility of the developed …

Development of a monoclonal-based ic-ELISA for the determination of kitasamycin in animal tissues and simulation studying its molecular recognition mechanism

L Li, R Hou, W Shen, Y Chen, S Wu, Y Wang, X Wang… - Food Chemistry, 2021 - Elsevier
To monitor the residue of kitasamycin (KIT), a monoclonal antibody against KIT was
prepared, and a 50% inhibition concentration (IC 50) of 5.7±1.4 μg/L was achieved with the …

IgYs: on her majesty's secret service

R Grzywa, A Łupicka-Słowik, M Sieńczyk - Frontiers in Immunology, 2023 - frontiersin.org
There has been an increasing interest in using Immunoglobulin Y (IgY) antibodies as an
alternative to “classical” antimicrobials. Unlike traditional antibiotics, they can be utilized on …

Single-chain variable fragment antibody-based ic-ELISA for rapid detection of macrolides in porcine muscle and computational simulation of its interaction mechanism

L Li, X Wang, R Hou, Y Wang, X Wang, C Xie, Y Chen… - Food Control, 2022 - Elsevier
To generate a single-chain fragment variable (scFv) for the detection of macrolide antibiotics
(MAs) residues, the biopanning, expression and comparative analysis of four scFvs against …

Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs

A Chorpunkul, U Boonyuen, K Limkittikul… - Scientific Reports, 2024 - nature.com
Animal rabies is a potentially fatal infectious disease in mammals, especially dogs.
Currently, the number of rabies cases in pet dogs is increasing in several regions of …

Docking guided phase display to develop fusion protein with novel scFv and alkaline phosphatase for one-step ELISA salbutamol detection

S Hu, G Yang, Z Chen, Q Li, B Liu, M Liu… - Frontiers in …, 2023 - frontiersin.org
Introduction Salbutamol (SAL) is a β2 adrenergic receptor agonist which has potential
hazardous effects for human health. It is very important to establish a sensitive and …

Development of an immunoaffinity chromatographic adsorbent using a moderate affinity, anti-salbutamol chicken scFv for high sensitivity LC-MS/MS detection of …

L Warren, SA Ali, CY Leow, SC Tan, CH Leow - Microchemical Journal, 2024 - Elsevier
Widespread misuse of salbutamol as an athletic performance enhancer and livestock growth
promoter necessitates its detection in biological matrices. Antibodies remain important …

Monoclonal IgY antibodies: advancements and limitations for immunodiagnosis and immunotherapy applications

AA Tabll, YE Shahein, MM Omran… - … in Vaccines and …, 2024 - journals.sagepub.com
Due to their high specificity and scalability, Monoclonal IgY antibodies have emerged as a
valuable alternative to traditional polyclonal IgY antibodies. This abstract provides an …

Heterologous antigen selection of chicken single-chain variable fragments against thiamethoxam

J He, Y Wang, D Wang, Y Yang, X Xue, T Xu, S Gu… - Analytical …, 2024 - pubs.rsc.org
Single-chain variable fragments (scFvs) are valuable in the development of immunoassays
for pesticide detection. In this study, scFvs specific to thiamethoxam (Thi) were successfully …