Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
What is the total number of protein molecules per cell volume? A call to rethink some published values
R Milo - Bioessays, 2013 - Wiley Online Library
Novel methods such as mass‐spectrometry enable a view of the proteomes of cells in
unprecedented detail. Recently, these efforts have culminated in quantitative measurements …
unprecedented detail. Recently, these efforts have culminated in quantitative measurements …
The quantitative and condition-dependent Escherichia coli proteome
Measuring precise concentrations of proteins can provide insights into biological processes.
Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art …
Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art …
Genome-wide inactivation of porcine endogenous retroviruses (PERVs)
The shortage of organs for transplantation is a major barrier to the treatment of organ failure.
Although porcine organs are considered promising, their use has been checked by …
Although porcine organs are considered promising, their use has been checked by …
[HTML][HTML] Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources
Quantitative views of cellular functions require precise measures of rates of biomolecule
production, especially proteins—the direct effectors of biological processes. Here, we …
production, especially proteins—the direct effectors of biological processes. Here, we …
Identification and characterization of essential genes in the human genome
Large-scale genetic analysis of lethal phenotypes has elucidated the molecular
underpinnings of many biological processes. Using the bacterial clustered regularly …
underpinnings of many biological processes. Using the bacterial clustered regularly …
Codon influence on protein expression in E. coli correlates with mRNA levels
Degeneracy in the genetic code, which enables a single protein to be encoded by a
multitude of synonymous gene sequences, has an important role in regulating protein …
multitude of synonymous gene sequences, has an important role in regulating protein …
Genomically recoded organisms expand biological functions
We describe the construction and characterization of a genomically recoded organism
(GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with …
(GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with …
Coordination of microbial metabolism
Beyond fuelling cellular activities with building blocks and energy, metabolism also
integrates environmental conditions into intracellular signals. The underlying regulatory …
integrates environmental conditions into intracellular signals. The underlying regulatory …
Quantitative proteomic analysis reveals a simple strategy of global resource allocation in bacteria
S Hui, JM Silverman, SS Chen, DW Erickson… - Molecular systems …, 2015 - embopress.org
A central aim of cell biology was to understand the strategy of gene expression in response
to the environment. Here, we study gene expression response to metabolic challenges in …
to the environment. Here, we study gene expression response to metabolic challenges in …