Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

Synthetic transcription factors (synTFs) that control beneficial transgene expression are an
important method to increase the safety and efficacy of cell and gene therapy. Reliance on …

CRISPR-based gene activation (CRISPRa) is a strategy for upregulating gene expression
by targeting promoters or enhancers in a tissue/cell-type specific manner. Here, we describe …

Cells rely on complex molecular networks to perceive and process external and internal
signals into tailored responses. These cellular abilities can be augmented and modified by …

Targeted epigenome editing tools allow precise manipulation and investigation of genome
modifications, however they often display high context dependency and variable efficacy …

To achieve exquisite control over the epigenome, we need a better predictive understanding
of how transcription factors, chromatin regulators, and their individual domain's function …

Repressive chromatin modifications are thought to compact chromatin to silence
transcription. However, it is unclear how chromatin structure changes during silencing and …

Epigenetic regulation involves the coordinated interplay of diverse proteins. To
systematically explore these combinations, we present COMBINE (combinatorial interaction …

The directed differentiation of stem cells into specific cell types is critical for regenerative
medicine and cell-based applications. However, current methods for cell fate control are …

Gene therapy holds great therapeutic potential. Yet, controlling cargo expression in single
cells is limited due to the variability of delivery methods. We implement an incoherent …