Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

TS Andrews, VY Kiselev, D McCarthy, M Hemberg - Nature protocols, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …

Astrocyte-immune cell interactions in physiology and pathology

RT Han, RD Kim, AV Molofsky, SA Liddelow - Immunity, 2021 - cell.com
Astrocytes play both physiological and pathological roles in maintaining central nervous
system (CNS) function. Here, we review the varied functions of astrocytes and how these …

The connectome of an insect brain

M Winding, BD Pedigo, CL Barnes, HG Patsolic, Y Park… - Science, 2023 - science.org
Brains contain networks of interconnected neurons and so knowing the network architecture
is essential for understanding brain function. We therefore mapped the synaptic-resolution …

Neuronal diversity and convergence in a visual system developmental atlas

MN Özel, F Simon, S Jafari, I Holguera, YC Chen… - Nature, 2021 - nature.com
Deciphering how neuronal diversity is established and maintained requires a detailed
knowledge of neuronal gene expression throughout development. In contrast to mammalian …

High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae

M Wang, Q Hu, T Lv, Y Wang, Q Lan, R Xiang, Z Tu… - Developmental Cell, 2022 - cell.com
Drosophila has long been a successful model organism in multiple biomedical fields. Spatial
gene expression patterns are critical for the understanding of complex pathways and …

The number of neurons in Drosophila and mosquito brains

JI Raji, CJ Potter - PLoS One, 2021 - journals.plos.org
Various insect species serve as valuable model systems for investigating the cellular and
molecular mechanisms by which a brain controls sophisticated behaviors. In particular, the …

A transcriptomic taxonomy of Drosophila circadian neurons around the clock

D Ma, D Przybylski, KC Abruzzi, M Schlichting, Q Li… - Elife, 2021 - elifesciences.org
Many different functions are regulated by circadian rhythms, including those orchestrated by
discrete clock neurons within animal brains. To comprehensively characterize and assign …

Decoding gene regulation in the fly brain

J Janssens, S Aibar, II Taskiran, JN Ismail, AE Gomez… - Nature, 2022 - nature.com
The Drosophila brain is a frequently used model in neuroscience. Single-cell transcriptome
analysis,,,,–, three-dimensional morphological classification and electron microscopy …

A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages

M Corrales, BT Cocanougher, AB Kohn… - Neural …, 2022 - Springer
Molecular profiles of neurons influence neural development and function but bridging the
gap between genes, circuits, and behavior has been very difficult. Here we used single cell …

A multilayer circuit architecture for the generation of distinct locomotor behaviors in Drosophila

AA Zarin, B Mark, A Cardona, A Litwin-Kumar, CQ Doe - Elife, 2019 - elifesciences.org
Animals generate diverse motor behaviors, yet how the same motor neurons (MNs)
generate two distinct or antagonistic behaviors remains an open question. Here, we …