The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …

In the last 10 years green fluorescent protein (GFP) has changed from a nearly unknown
protein to a commonly used tool in molecular biology, medicine, and cell biology. GFP is …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

Biomoleküle in ihrer natürlichen Umgebung zu erforschen, ist wegen der enormen
Komplexität zellulärer Systeme eine anspruchsvolle Aufgabe. In den letzten Jahren …

The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Abstract Changes in the interactions among proteins that participate in a biochemical
pathway can reflect the immediate regulatory responses to intrinsic or extrinsic perturbations …

▪ Abstract Inteins are genetic elements that disrupt the coding sequence of genes. However,
in contrast to introns, inteins are transcribed and translated together with their host protein …

We describe a new method for detecting protein− protein interactions in intact mammalian
cells; the approach is based on protein splicing-induced complementation of rationally …

We have developed a new unnatural amino acid based on the solvatochromic fluorophore 4-
N, N-dimethylamino-1, 8-naphthalimide (4-DMN) for application in the study of protein …

Investigations of the molecular processes that sustain life must include studies of these
processes in their normal cellular environment. The bimolecular fluorescence …