Genome editing using the clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated protein (Cas) gene-editing system (CRISPR-Cas) is a …

Genome editing technologies based on DNA-dependent polymerases (DDPs) could offer
several benefits compared with other types of editors to install diverse edits. Here, we …

Epigenetics refers to the molecules and mechanisms that modify gene expression states
without changing the nucleotide context. These modifications are what encode the cell state …

Abstract Dystrophy-associated fer-1-like protein (dysferlin) conducts plasma membrane
repair. Mutations in the DYSF gene cause a panoply of genetic muscular dystrophies. We …

Unintended on-target chromosomal alterations induced by CRISPR/Cas9 in mammalian
cells are common, particularly large deletions and chromosomal translocations, and present …

DNA methyltransferase is significant in cellular activities and gene expression, and its
aberrant expression is closely linked to various cancers during initiation and progression …

Gene knock-in can be defined as the introduction of precisely determined modifications,
insertions, or replacements to the genome, which enables the generation of reporter cells …

Background Recent studies have shown that, owning to its cohesive cleavage, Cas9-
mediated CRISPR gene editing outcomes at junctions of chromosomal rearrangements or …

The reprogramming of terminally differentiated cells over the past few years has become
important for induced pluripotent stem cells (iPSCs) in the field of regenerative medicine and …

Dystrophy-associated fer-1-like protein (dysferlin) conducts plasma membrane repair.
Mutations in the DYSF gene cause a panoply of genetic muscular dystrophies. We targeted …