Light sheet fluorescence microscopy
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
Scaffold-based 3D cell culture models in cancer research
Abstract Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer
research, offering significant advantages over traditional two-dimensional (2D) cell culture …
research, offering significant advantages over traditional two-dimensional (2D) cell culture …
Optical clearing methods: an overview of the techniques used for the imaging of 3D spheroids
EC Costa, DN Silva, AF Moreira… - Biotechnology and …, 2019 - Wiley Online Library
Spheroids have emerged as in vitro models that reproduce in a great extent the architectural
microenvironment found in human tissues. However, the imaging of 3D cell cultures is highly …
microenvironment found in human tissues. However, the imaging of 3D cell cultures is highly …
Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis
L Hof, T Moreth, M Koch, T Liebisch, M Kurtz, J Tarnick… - BMC biology, 2021 - Springer
Background Organoids are morphologically heterogeneous three-dimensional cell culture
systems and serve as an ideal model for understanding the principles of collective cell …
systems and serve as an ideal model for understanding the principles of collective cell …
Routine optical clearing of 3D-cell cultures: simplicity forward
E Nürnberg, M Vitacolonna, J Klicks… - Frontiers in Molecular …, 2020 - frontiersin.org
Three-dimensional cell cultures, such as spheroids and organoids, serve as increasingly
important models in fundamental and applied research and start to be used for drug …
important models in fundamental and applied research and start to be used for drug …
High-throughput deconvolution of 3D organoid dynamics at cellular resolution for cancer pharmacology with Cellos
Abstract Three-dimensional (3D) organoid cultures are flexible systems to interrogate
cellular growth, morphology, multicellular spatial architecture, and cellular interactions in …
cellular growth, morphology, multicellular spatial architecture, and cellular interactions in …
Organoid imaging: seeing development and function
R Keshara, YH Kim… - Annual review of cell and …, 2022 - annualreviews.org
Organoids are miniaturized and simplified versions of an organ produced in vitro from stem
or progenitor cells. They are used as a model system consisting of multiple cell types …
or progenitor cells. They are used as a model system consisting of multiple cell types …
A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models
Imaging and subsequent segmentation analysis in three-dimensional (3D) culture models
are complicated by the light scattering that occurs when collecting fluorescent signal through …
are complicated by the light scattering that occurs when collecting fluorescent signal through …
Applications and advances of multicellular tumor spheroids: challenges in their development and analysis
AG Mitrakas, A Tsolou, S Didaskalou… - International Journal of …, 2023 - mdpi.com
Biomedical research requires both in vitro and in vivo studies in order to explore disease
processes or drug interactions. Foundational investigations have been performed at the …
processes or drug interactions. Foundational investigations have been performed at the …
Recent trends and perspectives in cerebral organoids imaging and analysis
C Brémond Martin, C Simon Chane… - Frontiers in …, 2021 - frontiersin.org
Purpose: Since their first generation in 2013, the use of cerebral organoids has spread
exponentially. Today, the amount of generated data is becoming challenging to analyze …
exponentially. Today, the amount of generated data is becoming challenging to analyze …